‘Healed’ Experimental Gastric Ulcers Remain Histologically and Ultrastructurally Abnormal
‘Healed’ Experimental Gastric Ulcers Remain Histologically and Ultrastructurally Abnormal
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“治愈”的实验性胃溃疡在组织学和超微结构上仍然异常
DOI：
10.1097/00004836-199001001-00024
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发表时间：
1990
影响因子：
2.9
通讯作者：
H. Gergely
中科院分区：
文献类型：
--
作者：
A. Tarnawski;D. Hollander;W. Krause;W. Da̧broś;erzy Stachura;H. Gergely
本研究旨在评估 ATS 愈合溃疡（乙酸诱发的胃溃疡）区域胃粘膜的组织学和显微结构特征。我们研究的具体问题是溃疡严重愈合区域胃粘膜的结构和细胞组成是否完全恢复。对八十只布拉格-道利大鼠进行剖腹手术；将100%醋酸涂抹于下胃浆膜层30秒，关腹。两周后或2、3或4个月后重新打开胃。对来自严重愈合的溃疡区域的所有标准化胃样本进行获取、处理，并通过光学显微镜和透射电子显微镜进行评估。严重愈合的溃疡的胃粘膜在每个研究时间都显示出上皮细胞再生，但表面上皮下方的粘膜显示出显着的组织学和超微结构异常。可以区分两种不同模式的疤痕：（a）愈合溃疡区域的粘膜较薄（与正常相比减少25-45%），结缔组织增加，腺细胞分化差和/或退行性改变； (b) 粘膜显示胃腺呈气球状扩张、微血管网络减少以及陆地细胞分化不良。我们的结论是：（i）完全愈合的胃溃疡的上皮下粘膜显示腺体和血管结构紊乱，肾组织学和超微结构异常； (ii)这些异常可能干扰氧合、营养供应以及粘膜抵抗和防御，因此可能是溃疡复发的基础。
The present study was designed to assess histologic and iltrastructural features of gastric mucosa in the areas of rossly healed ulcers (acetic acid-induced gastric ulcers) in ats. The specific question we studied was whether the tructure and cellular composition of the gastric mucosa in n area of grossly healed ulcer were fully restored. Eighty prague-Dawley rats underwent laparotomy; 100% acetic cid was applied to the lower gastric corpus serosa for 30 s nd the abdomen was closed. The stomachs were reopened ter 2 weeks or after 2, 3, or 4 months. Standardized gastric all specimens from the area of grossly healed ulcers were btained, processed, and evaluated by light microscopy and y transmission electron microscopy. The gastric mucosa f grossly healed ulcers demonstrated re-epithelialization at ach study time but the mucosa beneath the surface epitheium displayed prominent histologic and ultrastructural abormalities. Two different patterns of scar could be distinuished: (a) the mucosa in the area of healed ulcer was hinner (25–45% reduction vs. normal), with increased onnective tissue and poor differentiation and/or degenerative changes in the glandular cells; or (b) the mucosa dislayed ballooning dilatation of gastric glands, reduction in he microvascular network, and poor differentiation of landular cells. We conclude that (i) the subepithelial muosa of grossly healed gastric ulcer displays disorganized estoration of glandular and vascular structures and renains histologically and ultrastructurally abnormal; (ii) hese abnormalities may interfere with oxygenation, nurient supply, and with mucosal resistance and defense, and herefore could be the basis for ulcer recurrence.