Seasonal localization of a collagenous protein in the organic matrix of spicules from the gorgonian Leptogorgia virgulata (Cnidaria: Gorgonacea)
Seasonal localization of a collagenous protein in the organic matrix of spicules from the gorgonian Leptogorgia virgulata (Cnidaria: Gorgonacea)
复制标题
柳珊瑚 Leptogorgia virgulata（刺胞动物门：Gorgonacea）针状体有机基质中胶原蛋白的季节性定位
作者：
R. J. Kingsley;J. Dupree
柳珊瑚Leptogorgia virgulata的针状体具有不溶性的基质部分，主要是胶原在夏季。这种胶原成分在冬季基本上不存在。使用针对140 kD的胶原蛋白（CP）的不溶性基质的抗体，免疫金标记被用来本地化这种蛋白质在透射电子显微镜水平全年，并在不同地区的柳珊瑚殖民地。在尖端区域内，140 kD CP全年变化的骨针，电子致密体（EDBs）的成硬结细胞，息肉囊泡，桥细胞和轴。在中部和基部，成骨细胞、成纤维细胞和轴的针状体、EDB和溶酶体中的140 kD CP全年变化。标记的位置和密度的这种变化表明不溶性基质的胶原成分的动态年度循环。EDB可以将基质的胶原成分运输到针状体形成空泡。140 kD CP的组分可分别由息肉囊泡和溶酶体转运和/或降解。轴向区域的标记模式表明可能发生胶原蛋白组分的移位和储存。环境因素可能是触发基质循环的原因。
Spicules of the gorgonian Leptogorgia virgulata possess an insoluble matrix fraction that is predominantly collagenous in summer months. This collagenous component is largely absent in winter months. Using an antibody directed against the 140 kD collagenous protein (CP) of the insoluble matrix, immuno-gold labelling was employed to localized this protein at the transmission electron-microscopy level throughout the year, and in different areas of the gorgonian colonies. Within the tip regions, the 140 kD CP varied throughout the year in the spicules, electron-dense bodies (EDBs) of scleroblasts, polyp vesicles, desmocytes and axes. In the mid and base regions, the 140 kD CP varied throughout the year in the spicules, EDBs and lysosomes of scleroblasts, desmocytes and axes. This variation in the location and density of the label suggests a dynamic annual cycling of the collagenous component of the insoluble matrix. EDBs may transport a collagenous component of the matrix to the spicule-forming vacuole. A component of the 140 kD CP may be transported and/or degraded by polyp vesicles and lysosomes, respectively. The pattern of labelling of the axial region suggests that translocation and storage of a component of the collagenous protein may occur. Environmental factors may be responsible for the triggering of matrix cycling.