Transformation of chicken cells by the gene encoding the catalytic subunit of PI 3-kinase
Transformation of chicken cells by the gene encoding the catalytic subunit of PI 3-kinase
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DOI：
10.1126/science.276.5320.1848
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发表时间：
1997-06-20
期刊：
影响因子：
56.9
通讯作者：
Vogt, PK
中科院分区：
文献类型：
--
作者：
Chang, HW;Aoki, M;Vogt, PK
禽肉瘤病毒16（ASV 16）是一种逆转录病毒，可诱导鸡血管瘤。对ASV 16基因组的分析显示，它编码一种致癌基因，该基因来源于磷酸肌醇3-激酶（PI 3-激酶）催化亚基的细胞基因。该基因被称为v-p3 k，与其细胞对应物c-p3 k一样，它是培养的鸡胚成纤维细胞（CEF）中的有效转化基因。病毒和细胞p3 k基因的产物具有PI 3-激酶活性。用任一基因转化的CEF显示升高的磷脂酰肌醇3，4-二磷酸和磷脂酰肌醇3，4，5-三磷酸水平以及Akt激酶的活化。
The avian sarcoma virus 16 (ASV 16) is a retrovirus that induces hemangiosarcomas in chickens. Analysis of the ASV 16 genome revealed that it encodes an oncogene that is derived from the cellular gene for the catalytic subunit of phosphoinositide 3-kinase(PI 3-kinase). The gene is referred to as v-p3k, and like its cellular counterpart c-p3k, it is a potent transforming gene in cultured chicken embryo fibroblasts (CEFs). The products of the viral and cellular p3k genes have PI 3-kinase activity. CEFs transformed with either gene showed elevated levels of phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate and activation of Akt kinase.