A subpopulation of CD24+ cells in colon cancer cell lines possess stem cell characteristics
A subpopulation of CD24+ cells in colon cancer cell lines possess stem cell characteristics
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DOI：
10.4149/neo_2012_036
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发表时间：
2012-01-01
期刊：
影响因子：
3
通讯作者：
Lan, P.
中科院分区：
文献类型：
--
作者：
Ke, J.;Wu, X.;Lan, P.
癌症干细胞 (CSC) 已被证明有助于乳腺癌和神经胶质瘤等一系列癌症类型的抵抗和复发。然而，结肠癌干细胞的特征仍知之甚少。在此我们报道结肠癌细胞系HCT116和SW480中的CD24(+)亚群表现出癌症干细胞样特征。在结肠癌细胞系 HCT116、SW480 和 HT29 上对来自其他类型癌症的已知 CSC 标记进行初步筛选后，使用流式细胞术选择候选 CSC 标记。 CD24 在 HCT116 和 SW480 细胞系的少数大量细胞群中表达。此外，功能测试表明，与CD24(-)亚群相比，CD24(+)细胞在体外和体内表现出增强的化疗耐药性、自我更新和致瘤能力。这些结果表明结肠癌细胞系HCT116和SW480中的CD24(+)亚群表现出类似CSC的特征，并且代表了研究和开发克服结肠癌化疗耐药和复发的有效策略的良好模型。
Cancer stem cells (CSCs) have been shown to contribute to the resistance and relapse in a range of cancer types such as breast cancer and glioma. However, colon cancer stem cells remain poorly characterized. Here we reported that CD24(+) subpopulation in colon cancer cell lines HCT116 and SW480 exhibited cancer stem cell-like characteristics. Using flow cytometry candidate CSCs markers were selected after initial screening of known CSCs markers from other types of cancer on colon cancer cell lines HCT116, SW480 and HT29. CD24 was expressed in the minority of bulk cell population of HCT116 and SW480 cell lines. Moreover, functional tests demonstrated that CD24(+) cells exhibited enhanced chemotherapy-resistance, self-renewal and tumorigenic capacity both in vitro and in vivo, compared to CD24(-) subpopulations. These results suggest that CD24(+) subpopulation in colon cancer cell lines HCT116 and SW480 exhibits CSCs like characteristics, and represents a nice model to study and develop effective strategies to overcome chemo-resistance and relapse of colon cancer.