Evaluation of diagnostic measures for subacute ruminal acidosis in dairy cows
Evaluation of diagnostic measures for subacute ruminal acidosis in dairy cows
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DOI：
10.4141/cjas2012-004
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发表时间：
2012-09-01
影响因子：
1
通讯作者：
Plaizier, J. C.
中科院分区：
文献类型：
--
作者：
Li, S.;Gozho, G. N.;Plaizier, J. C.
Li， S., Gozho， G. N., Gakhar， N., Khafipour， E., Krause， D.和Plaizier， J. C. 2012。奶牛亚急性瘤胃酸中毒诊断措施的评价。J.阿尼姆。科学学报，42(2):353-364。研究了亚急性瘤胃酸中毒（SARA）对粪便、尿液、牛奶和血液样本测量以及摄食行为的影响，以确定哪些测量可能有助于SARA的诊断。选取8头产乳奶牛，采用交叉设计，分2个6周试验期。第1、2、6周饲喂料精比为58:42的对照饲粮。在第3 ~ 5周，分别用含有50%磨碎小麦和50%磨碎大麦的颗粒代替对照日粮中12%的干物质，用含有26%磨碎苜蓿的颗粒代替对照日粮中26%的干物质，进行以谷物为基础的SARA攻击（GBSC）或苜蓿颗粒SARA攻击（APSC）。瘤胃pH值降低在不同的挑战之间没有差异。GBSC增加了粪便中脂多糖（LPS）和血液中血清淀粉样蛋白A的浓度，降低了血液中乳脂和尿素的浓度。APSC增加了尿pH值、净酸碱排泄量、红细胞计数和血钾浓度。两种处理均提高了瘤胃液中脂多糖和丙酸的浓度、乳中蛋白质、葡萄糖、乳酸和钠的浓度以及血液中CO2的分压，并有降低血液中氯离子浓度的趋势。同样受到这两种挑战影响的测量可能有助于诊断瘤胃pH值下降。SARA挑战之间的差异表明这种疾病不仅仅依赖于瘤胃pH。
Li, S., Gozho, G. N., Gakhar, N., Khafipour, E., Krause, D. O. and Plaizier, J. C. 2012. Evaluation of diagnostic measures for subacute ruminal acidosis in dairy cows. Can J. Anim. Sci. 92: 353-364. Effects of subacute ruminal acidosis (SARA) challenges on measurements of feces, urine, milk and blood samples, and of feeding behavior were investigated to determine which of these measurements may aid in the diagnosis of SARA. Eight multiparous lactating dairy cows were used in a crossover design with two 6-wk experimental periods. During weeks 1,2, and 6, cows received a control diet with a forage-to-concentrate ratio of 58:42. During weeks 3 to wk 5, a grain-based SARA challenge (GBSC) or an alfalfa-pellet SARA challenge (APSC) was conducted by replacing 12% of the dry matter of the control ration with pellets containing 50% ground wheat and 50% ground barley, and by replacing 26% of the dry matter of the control ration with pellets of ground alfalfa, respectively. The rumen pH depression did not differ between the challenges. The GBSC increased the concentrations of lipopolysaccharide (LPS) in feces and of serum amyloid A in blood, but decreased that of milk fat and urea in blood. The APSC increased the urine pH, the net-acid-base excretion, and the red blood cell count and potassium concentration in blood. Both challenges increased the concentrations of LPS and propionate in rumen fluid, protein in milk, glucose, lactate and sodium and the partial pressure of CO2 in blood, and tended to decrease the concentration of chloride in blood. The measures that were similarly affected by both challenges may aid in the diagnosis of a rumen pH depression. Differences between the SARA challenges suggest that this disorder is not solely rumen pH dependent.