Porcine sialoadhesin suppresses type I interferon production to support porcine reproductive and respiratory syndrome virus infection
Porcine sialoadhesin suppresses type I interferon production to support porcine reproductive and respiratory syndrome virus infection
复制标题
猪唾液酸粘附素抑制 I 型干扰素产生以支持猪繁殖与呼吸综合征病毒感染
DOI：
10.1186/s13567-020-00743-7
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发表时间：
2020-02-24
影响因子：
4.4
通讯作者：
Zhang, Gaiping
中科院分区：
文献类型：
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作者：
Liu, Yingqi;Li, Rui;Zhang, Gaiping
猪繁殖与呼吸综合征病毒（Porcine reproductive and respiratory syndrome virus，PRRSV）是对全球养猪业的重大威胁。猪唾液酸粘附素（poSn）先前已显示介导PRRSV附着和内化。在目前的研究中，我们报告了其在对抗I型干扰素（IFN）的生产过程中的PRRSV感染的身份不明的作用。我们确定poSn通过抑制I型IFN转录促进PRRSV感染。在机制上，poSn与12 kDa的DNA激活蛋白（DAP 12）相互作用，这是依赖于DAP 12跨膜结构域（TMD）内的残基51-57。PRRSV利用poSn-DAP 12途径减弱核因子-κ B（NF-κB）的活化。更重要的是，poSn-DAP 12通路参与抑制poly（I：C）触发的IFN产生。所有这些结果揭示了poSn在抑制宿主抗病毒反应中的新作用，加深了我们对PRRSV发病机制的理解。
Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant threat to the global swine industry. Porcine sialoadhesin (poSn) has been previously shown to mediate PRRSV attachment and internalization. In the current study, we report its unidentified role in antagonism of type I interferon (IFN) production during PRRSV infection. We determined that poSn facilitated PRRSV infection via inhibition of type I IFN transcription. Mechanistically, poSn interacted with a 12 kDa DNAX-activation protein (DAP12), which was dependent on residues 51–57 within DAP12 transmembrane domain (TMD). PRRSV exploited the poSn-DAP12 pathway to attenuate activation of nuclear factor-kappa B (NF-κB). More importantly, the poSn-DAP12 pathway was involved in inhibiting poly (I:C)-triggered IFN production. All these results reveal a novel role of poSn in suppressing host antiviral responses, which deepens our understanding of PRRSV pathogenesis.