Liquid chromatographic determination of toxigenic secondary metabolites produced by Fusarium strains
Liquid chromatographic determination of toxigenic secondary metabolites produced by Fusarium strains
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DOI：
10.1016/s0021-9673(02)00214-5
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发表时间：
2002-05-10
影响因子：
4.1
通讯作者：
Jiménez, M
中科院分区：
文献类型：
--
作者：
Mateo, JJ;Mateo, R;Jiménez, M
本文对镰刀菌产生的真菌毒素（玉米赤霉烯酮、腐殖酸和伏马菌素）的液相色谱分析方法进行了比较。结果表明，现代的清洁程序，采用多功能MycoSep和免疫亲和柱的适用性，虽然这些方法是更昂贵的比传统的清洁方法。高效液相色谱荧光和光电二极管阵列检测是一个合适的技术用于分析有毒的次生代谢产物产生的镰刀菌物种;不同的衍生化策略进行了研究，以提高灵敏度的技术，因为这些代谢产物在污染的食品中的浓度低。所提出的方法的效用进行了评估，在谷物文化的各种镰刀菌菌株。(C)2002 Elsevier Science B. V.保留所有权利。
Various liquid chromatographic methods used in the analysis of mycotoxins (zearalenone, trichothecenes and fumonisins) produced by Fusarium species were compared in this work. The results demonstrate the suitability of modem clean-up procedures employing multi functional MycoSep and immunoaffinity columns although these methods are more expensive than conventional methodologies for clean-up. HPLC with both fluorescence and photodiode array detection is a suitable technique for the analysis of toxic secondary metabolites produced by Fusarium species; different derivatisation strategies have been studied to improve the sensitivity of the technique because of the low concentration of these metabolites in contaminated food. The utility of the proposed methodology was assessed in cereal cultures of various Fusarium strains. (C) 2002 Elsevier Science B.V. All rights reserved.