Catechin is present as metabolites in human plasma after consumption of red wine
Catechin is present as metabolites in human plasma after consumption of red wine
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DOI：
10.1093/jn/129.9.1662
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发表时间：
1999-09-01
影响因子：
4.2
通讯作者：
Waterhouse, AL
中科院分区：
文献类型：
--
作者：
Donovan, JL;Bell, JR;Waterhouse, AL
类黄酮是水果、蔬菜和酒的成分。饮食中丰富的黄酮类化合物与降低心脏病死亡率相关，表明它们具有保护作用，然而，人们对正常食物摄入后黄酮类化合物的吸收和代谢知之甚少，本研究测量了五名男性和四名女性志愿者每天饮用120毫升红葡萄酒（RW）和另外一天饮用去醇红葡萄酒（DRW）后血浆中丰富的黄酮类化合物(+)-儿茶素及其代谢物的水平。每个葡萄酒样品含有35 +/- 1 mg儿茶素（平均+/- SEM），儿茶素及其代谢物3'- o -甲基儿茶素（3' mc）的血浆水平采用三甲基silylated （TMS）衍生物的气相色谱-质谱（GC-MS）测定。酶解后测定葡萄糖醛酸盐和硫酸盐的缀合物。在服用RW或DRW前，儿茶素、3′mc和所有结合物的血浆水平均为
Flavonoids are components of fruits, vegetables and wines. An abundance of flavonoids in the diet is correlated with reduced heart disease mortality, suggesting that they act as protective nutrients, However, little is known about the absorption and metabolism of flavonoids after normal foods are consumed, This study measured the levels of one abundant flavonoid, (+)-catechin, and its metabolites in plasma after five male and four female volunteers consumed 120 mL of red wine (RW) one day and de-alcoholized red wine (DRW) on a separate day. Each wine sample contained 35 +/- 1 mg catechin (mean +/- SEM), Plasma levels of catechin and its metabolite 3'-O-methylcatechin (3'MC) were determined by gas chromatography-mass spectrometry (GC-MS) of the trimethylsilylated (TMS) derivatives. Glucuronide and sulfate conjugates were determined after enzymatic hydrolysis. Before RW or DRW consumption, plasma levels of catechin, 3'MC and all conjugates were