In situ laser manipulation of root tissues in transparent soil.
In situ laser manipulation of root tissues in transparent soil.
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DOI：
10.1007/s11104-021-05133-2
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发表时间：
2021
期刊：
影响因子：
4.9
通讯作者：
MacDonald MP
中科院分区：
文献类型：
--
作者：
Ge S;Dupuy LX;MacDonald MP
激光显微操作，如解剖或光学捕获，使远程物理修改的组织，细胞和细胞器的活动。迄今为止，激光操纵在土壤中生长的植物根的应用受到限制。在这里，我们展示了当植物根生长在透明土壤中时，激光操作可以原位应用。我们已经开发了一个Q开关激光操纵和成像仪器进行控制解剖的根，并研究光诱导的根生长反应。我们进行了详细的特性的切割光束通过土壤，研究解剖和光学烧蚀。此外，我们还研究了在空气、琼脂、凝胶和透明土中利用低光剂量控制莴苣幼苗的根伸长速率。我们表明，虽然土壤的不均匀性影响的梁的厚度和圆度，这些扭曲并不是固有的限制。诱导根伸长或根的微观区域的完全解剖的变化的能力对于基质异质性和显微镜设置是稳健的，并且在透明土壤环境诱导的有限扭曲之后保持。我们的研究结果表明，控制在原位激光切割的根组织是可能的，一个简单的和低成本的光学设置。我们还表明，在没有解剖，减少激光功率密度可以提供可逆的控制根的生长，实现了精确的“点和拍摄”的方法根操作。
Laser micromanipulation such as dissection or optical trapping enables remote physical modification of the activity of tissues, cells and organelles. To date, applications of laser manipulation to plant roots grown in soil have been limited. Here, we show laser manipulation can be applied in situ when plant roots are grown in transparent soil. We have developed a Q-switched laser manipulation and imaging instrument to perform controlled dissection of roots and to study light-induced root growth responses. We performed a detailed characterisation of the properties of the cutting beams through the soil, studying dissection and optical ablation. Furthermore, we also studied the use of low light doses to control the root elongation rate of lettuce seedlings (Lactuca sativa) in air, agar, gel and transparent soil. We show that whilst soil inhomogeneities affect the thickness and circularity of the beam, those distortions are not inherently limiting. The ability to induce changes in root elongation or complete dissection of microscopic regions of the root is robust to substrate heterogeneity and microscopy set up and is maintained following the limited distortions induced by the transparent soil environment. Our findings show that controlled in situ laser dissection of root tissues is possible with a simple and low-cost optical set-up. We also show that, in the absence of dissection, a reduced laser light power density can provide reversible control of root growth, achieving a precise “point and shoot” method for root manipulation.
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