QTL mapping for combining ability in different population-based NCII designs: a simulation study
QTL mapping for combining ability in different population-based NCII designs: a simulation study
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不同群体 NCII 设计中组合能力的 QTL 作图：模拟研究
DOI：
10.1007/s12041-013-0311-6
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发表时间：
2013-12-01
影响因子：
1.5
通讯作者：
Hu, Zhongli
中科院分区：
文献类型：
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作者：
Li, Lanzhi;Sun, Congwei;Hu, Zhongli
NCII设计（北卡罗来纳州交配设计II）在配合力和杂种优势研究中得到广泛应用。本研究的目的是估计不同的基础群体、样本大小、测交数和遗传力对配合力和杂种优势QTL分析的影响。然后对基础群体表现、测交群体表型值、一般配合力（GCA）、特殊配合力（SCA）和中亲优势（Hmp）数据集进行了一系列Monte Carlo模拟试验和QTL定位。结果表明：（1）增加测验人数并不一定能提高QTL的检测能力，但与QTL效应显著相关。(ii)在基础群体中检测到的QTL可能与GCA数据库中检测到的QTL不同。从SCA和Hmp数据集中检测到的QTL中也可以看到类似的现象。(iii)在遗传力较低的情况下，QTL对一般配合力的检测能力大小顺序为DH（RIL）设计> F2设计> BC设计。重组自交系（RILs）（或DH）允许更多的重组，并提供比其他群体更高的定位分辨率。此外，它们的测交后代可以重复产生并进行表型分析。因此，建议采用基于RIL（或DH）的NCII设计进行配合力QTL分析。本研究结果可为选育高配合力优良亲本及遗传学家研究配合力的遗传基础提供参考。
The NCII design (North Carolina mating design II) has been widely applied in studies of combining ability and heterosis. The objective of our research was to estimate how different base populations, sample sizes, testcross numbers and heritability influence QTL analyses of combining ability and heterosis. A series of Monte Carlo simulation experiments with QTL mapping were then conducted for the base population performance, testcross population phenotypic values and the general combining ability (GCA), specific combining ability (SCA) and Hmp (midparental heterosis) datasets. The results indicated that: (i) increasing the number of testers did not necessarily enhance the QTL detection power for GCA, but it was significantly related to the QTL effect. (ii) The QTLs identified in the base population may be different from those from GCA dataset. Similar phenomena can be seen from QTL detected in SCA and Hmp datasets. (iii) The QTL detection power for GCA ranked in the order of DH(RIL) based > F2based > BC based NCII design, when the heritability was low. The recombinant inbred lines (RILs) (or DHs) allows more recombination and offers higher mapping resolution than other populations. Further, their testcross progeny can be repeatedly generated and phenotyped. Thus, RIL based (or DH based) NCII design was highly recommend for combining ability QTL analysis. Our results expect to facilitate selecting elite parental lines with high combining ability and for geneticists to research the genetic basis of combining ability.