Molecular survey of Babesia infection in dogs in Okinawa, Japan
Molecular survey of Babesia infection in dogs in Okinawa, Japan
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DOI：
10.1016/j.vetpar.2004.03.012
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发表时间：
2004-05-26
影响因子：
2.6
通讯作者：
Hirakawa, M
中科院分区：
文献类型：
--
作者：
Inokuma, H;Yoshizaki, Y;Hirakawa, M
通过聚合酶链式反应 (PCR) 和序列分析，对日本冲绳岛总共 80 只自由漫游的狗进行了巴贝虫感染检查。在 80 个样本中，有 12 个在巴贝虫属特异性 PCR 中呈阳性。随后对犬巴氏杆菌和吉氏巴氏杆菌进行的物种特异性 PCR 显示，分别有 5 只 (6.3%) 和 7 只 (8.8%) 狗感染了犬巴氏杆菌和吉氏巴氏杆菌。 PCR产物序列分析显示，冲绳犬犬小孢子虫18S rRNA基因序列与沃氏犬小孢子虫非常接近，序列相似性达99.94%。 (C) 2004 Elsevier B.V. 保留所有权利。
A total of 80 free-roaming dogs on Okinawa Island, Japan, were examined for Babesia infection using the polymerase chain reaction (PCR) and sequence analysis. Of 80 samples, 12 were positive in a Babesia genus-specific PCR. Consequent species- specific PCR for B. canis and B. gibsoni revealed that 5 (6.3%) and 7 (8.8%) dogs were infected with B. canis and B. gibsoni, respectively. Sequence analysis of the PCR products revealed that the 18S rRNA gene sequence of B. canis detected from dogs in Okinawa was very close to B. canis vogeli with sequence similarity of 99.94%. (C) 2004 Elsevier B.V. All rights reserved.