Differentiation between Actinobacillus (Haemophilus) actinomycetemcomitans, Haemophilus aphrophilus and Haemophilus paraphrophilus by multilocus enzyme electrophoresis.
Differentiation between Actinobacillus (Haemophilus) actinomycetemcomitans, Haemophilus aphrophilus and Haemophilus paraphrophilus by multilocus enzyme electrophoresis.
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通过多位点酶电泳区分放线杆菌（嗜血杆菌）放线菌、嗜血嗜血杆菌和副嗜血杆菌。
DOI：
10.1099/00221287-136-10-2135
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Olsen,I
中科院分区：
文献类型：
--
作者：
Caugant,DA;Selander,RK;Olsen,I
伴生放线杆菌、嗜人嗜血杆菌和H。通过对编码代谢酶的14个结构基因进行电泳性等位基因变异分析，确定了副嗜血杆菌。在所分析的51个菌株中，有25个电泳型，平均每个座位的遗传多样性为0.753。ETS的聚类分析显示，一组明确的11个ETS仅代表所有17个被指定为ToA的分离物的基因类型。其余14个Et代表34株H。性欲和H。甲嗜性。除ATCC 13252外，所有的H。亲性株与亲缘关系密切，而菌株归属为ToH。副亲缘关系包括远亲谱系，其中一些与Ofh相似。Phrophusand应归属于本种。因此，研究表明A和A之间总体上没有显著的遗传相似性。伴生放线菌和两株嗜血杆菌。
Genetic relationships among isolates assigned toActinobacillus actinomycetemcomitans, Haemophilus aphrophilusandH. paraphrophiluswere determined by analysis of electrophoretically demonstrable allelic variation in 14 structural genes encoding metabolic enzymes. Among the 51 isolates analysed there were 25 electrophoretic types (ETs), among which mean genetic diversity per locus was 0·753. Cluster analysis of ETs demonstrated one well-defined group of 11 ETs representing solely the genotypes of all 17 isolates assigned toA. actinomycetemcomitans.The remaining 14 ETs represented the genotypes of the 34 isolates ofH. aphrophilusandH. paraphrophilus.With the exception of ATCC 13252, all strains ofH. aphrophiluswere closely related, whereas strains assigned toH. paraphrophilusincluded distantly related lineages, some of which were similar to those ofH. aphrophilusand should be assigned to this species. Thus, the study showed that there is no significant overall genetic similarity betweenA. actinomycetemcomitansand the twoHaemophilusspp.