Expression of a family of noncoding mitochondrial RNAs distinguishes normal from cancer cells
Expression of a family of noncoding mitochondrial RNAs distinguishes normal from cancer cells
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DOI：
10.1073/pnas.0903086106
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发表时间：
2009-06-09
影响因子：
11.1
通讯作者：
Burzio, Luis O.
中科院分区：
文献类型：
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作者：
Burzio, Veronica A.;Villota, Claudio;Burzio, Luis O.
我们报道了在人类细胞中存在的非编码线粒体RNA，其含有共价连接到线粒体16S RNA（16S mtrRNA）的5'末端的815个核苷酸（nt）的反向重复（IR）。该转录物含有茎环结构，并在人增殖细胞中表达，但不在静息细胞中表达。在这里，我们证明，除了该转录本之外，培养中的正常人类增殖细胞还表达2种反义线粒体转录本。这些转录物也含有茎环结构，但引人注目的是，它们在17种不同肿瘤类型的肿瘤细胞系和肿瘤细胞中下调。这些转录本的差异表达将正常细胞与肿瘤细胞区分开来，并可能对癌症生物学和诊断学产生独特的见解。
We reported the presence in human cells of a noncoding mitochondrial RNA that contains an inverted repeat (IR) of 815 nucleotides (nt) covalently linked to the 5' end of the mitochondrial 16S RNA (16S mtrRNA). The transcript contains a stem-loop structure and is expressed in human proliferating cells but not in resting cells. Here, we demonstrate that, in addition to this transcript, normal human proliferating cells in culture express 2 antisense mitochondrial transcripts. These transcripts also contain stem-loop structures but strikingly they are down-regulated in tumor cell lines and tumor cells present in 17 different tumor types. The differential expression of these transcripts distinguishes normal from tumor cells and might contribute a unique vision on cancer biology and diagnostics.