Silencing GhCOI1 in Gladiolus hybridus increases susceptibility to Alternaria brassicicola and impairs inducible defenses
Silencing GhCOI1 in Gladiolus hybridus increases susceptibility to Alternaria brassicicola and impairs inducible defenses
复制标题
沉默唐菖蒲中的 GhCOI1 会增加对芸苔链格孢菌的易感性并损害诱导防御
DOI：
10.1007/s11240-019-01711-6
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Yi Mingfang
中科院分区：
文献类型：
--
作者：
Seng Shanshan;Wu Chenyu;Wu Jian;Zhong Xionghui;He Junna;Yi Mingfang
芸苔链格孢（Alternaria brassicicolais）是一种严重影响唐菖蒲切花和球茎产量和品质的坏死性病原真菌。茉莉酸信号通路在植物抗病性中起着重要作用。COI1蛋白是该途径的关键调节因子，COI1基因可以调节茉莉酸信号诱导的对坏死性真菌的防御。本研究对唐菖蒲属植物GhCOI 1基因进行了研究，发现GhCOI 1在叶片中的表达量最高，其次是根。低浓度的MeJA促进GhCOI 1的表达，6 h后仍保持较高水平。通过VIGS沉默GhCOI 1降低了GhCOI 1的转录水平。真菌菌丝的长度和真菌DNA的量在沉默植物中显着大于对照植物。此外，GhCOI 1在唐菖蒲对A. GhCOI 1沉默减少了H2O2的积累，使真菌通过气孔进入叶片组织，直接引起叶片坏死。综上所述，这些结果表明，GhCOI1是一个重要的信号传导组件，控制JA调节的防御反应againstA。芸苔属剑兰属植物。
Alternaria brassicicolais a necrotrophic pathogenic fungus that severely affects both the yield and quality ofGladioluscut flowers and cormels. The jasmonate signaling pathway plays an important role in plant disease resistance. The protein CORONATINE INSENSITIVE 1 (COI1) is a key regulator of this pathway, and theCOI1gene can modulate jasmonate signaling-induced defenses against necrotrophic fungi. Here, we characterize aCOI1gene fromGladiolus,GhCOI1.GhCOI1transcript levels were greatest in the leaves, followed by the roots. A low MeJA concentration promotedGhCOI1expression, which remained high after 6 h. SilencingGhCOI1via VIGS reducedGhCOI1transcript levels. The length of fungal hyphae and amount of fungal DNA were significantly greater in silenced plants than in control plants. Moreover, GhCOI1 was necessary for the induction of SOD, POD, PPO, and CAT in theGladiolusdefense response toA. brassicicolainfestation.GhCOI1silencing reduced the accumulation of H2O2, allowing the fungus to enter leaf tissue through stomata and cause necrosis directly. Taken together, these results suggest that GhCOI1 is an essential signaling component that controls the JA-regulated defense response againstA. brassicicolainGladiolusplants.