Age, sex, and lactating status regulate ghrelin secretion and GOAT mRNA levels from isolated rat stomach
Age, sex, and lactating status regulate ghrelin secretion and GOAT mRNA levels from isolated rat stomach
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DOI：
10.1152/ajpendo.00057.2010
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发表时间：
2010-09-01
影响因子：
5.1
通讯作者：
Seoane, L. M.
中科院分区：
文献类型：
--
作者：
Al-Massadi, O.;Crujeiras, A. B.;Seoane, L. M.
阿尔-马萨迪 O、克鲁热拉斯 AB、冈萨雷斯 RC、帕尔多 M、迪格斯 C、卡萨努埃瓦 FF、Seoane LM。年龄、性别和哺乳状态调节离体大鼠胃中的生长素释放肽分泌和山羊 mRNA 水平。 Am J Physiol Endocrinol Metab 299：E341-E350，2010。首次发表于 2010 年 5 月 25 日； doi:10.1152/ajpendo.00057.2010.-Ghrelin 是一种参与能量稳态调节的胃衍生肽，而 ghrelin O-酰基转移酶 (GOAT) 是负责 ghrelin 酰化的酶。青春期是一个代谢需求发生深刻变化和性激素水平显着变化的时期。另一方面，断奶过程是饮食的根本改变，这涉及胃肠道生理学的一些适应。迄今为止，尚未研究过在这些条件下胃直接分泌生长素释放肽而不受其他器官的干扰。本文的主要目的是研究胃如何调节生长素释放肽的产生和分泌以及这些生命时期的 GOAT 表达。胃生长素释放肽的分泌在出生后以独立的胃表达和该激素的循环水平的方式进行调节。目前的工作表明雌激素对胃生长素释放肽的分泌有很强的调节作用。断奶强烈调节胃饥饿素的分泌。延迟断奶的动物体重低于相应的对照动物。首次表明，睾酮和雌激素循环水平的显着下降与断奶延迟有关。胃中 GOAT mRNA 水平受到年龄、母乳喂养和睾酮的强烈调节。总之，胃本身调节生长素释放肽和 GOAT 的产生，以使生物体适应生命各个阶段所需的代谢需求。
Al-Massadi O, Crujeiras AB, Gonzalez RC, Pardo M, Dieguez C, Casanueva FF, Seoane LM. Age, sex, and lactating status regulate ghrelin secretion and goat mRNA levels from isolated rat stomach. Am J Physiol Endocrinol Metab 299: E341-E350, 2010. First published May 25, 2010; doi:10.1152/ajpendo.00057.2010.-Ghrelin is a stomach derivate peptide involved in energy homeostasis regulation, and ghrelin O-acyltransferase (GOAT) is the enzyme responsible for ghrelin acylation. Puberty is a period characterized by profound changes in the metabolic requirements and notable variations of sexual hormone levels. On the other hand, the weaning process is a fundamental modification of the diet, which implicates several adaptations of the gastrointestinal tract physiology. Until now the direct secretion of ghrelin by the stomach in these conditions, without interferences from other organs, has never been studied. The main objective of this article was to investigate how the stomach modulates ghrelin production and secretion as well as GOAT expression on these periods of life. Gastric ghrelin secretion is regulated through postnatal life in an independent way of gastric expression and circulating levels of this hormone. The present work shows a strong regulation of gastric ghrelin secretion by estrogens. The weaning strongly regulates gastric ghrelin secretion. Animals subjected to delayed weaning present a lower body weight than the corresponding controls. For the first time, it is shown that a noticeable decrease in circulating levels of testosterone and estrogens is associated with delay of weaning. GOAT mRNA levels in the stomach are strongly regulated by age, breastfeeding, and testosterone. In conclusion, the stomach itself regulates ghrelin and GOAT production to adapt the organism to the metabolic requirements demanded through each stage of life.