Multimodal optical microscope for detecting viability of mouse embryos in vitro.
Multimodal optical microscope for detecting viability of mouse embryos in vitro.
复制标题
用于检测小鼠胚胎体外活力的多模态光学显微镜。
DOI：
--
复制
发表时间：
2007
影响因子：
3.5
通讯作者：
C. DiMarzio
中科院分区：
文献类型：
--
作者：
W. Warger;G. Laevsky;D. Townsend;M. Rajadhyaksha;C. DiMarzio
我们提出了一种多模态光学显微镜，它在一个共同的平台上集成了六种成像方式。成像方式包括三种凝视模式，光学正交显微镜（OQM），微分干涉对比（DIC）显微镜，和落射荧光显微镜，和三种扫描模式，共焦反射显微镜（CRM），共焦荧光显微镜（CFM），和双光子显微镜（2PM）。OQM重建的幅度和相位的光学透明的标本内修改的马赫-曾德尔配置。DIC显微镜成像的相位梯度沿着一个特定方向的光学透明的标本。CRM检测折射率的变化，调制后向散射。落射荧光显微镜、CFM和2PM检测样本内的内源性和外源性荧光团。由于光学切片和局部探测，扫描模式固有地能够产生三维（3-D）图像。照明和成像是同轴进行的，模式之间的光学组件的变化最小。胚胎的多模态图像展示了显微镜的成像能力。
We present a multimodal optical microscope that incorporates six imaging modalities on one common platform. The imaging modalities include three staring modes, optical quadrature microscopy (OQM), differential interference contrast (DIC) microscopy, and epi-fluorescence microscopy, and three scanning modes, confocal reflectance microscopy (CRM), confocal fluorescence microscopy (CFM), and two-photon microscopy (2PM). OQM reconstructs the amplitude and phase of an optically transparent specimen within a modified Mach-Zehnder configuration. DIC microscopy images the phase gradient along a specified direction of an optically transparent specimen. CRM detects index of refraction changes that modulate backscatter. Epi-fluorescence microscopy, CFM, and 2PM detect endogenous and exogenous fluorophores within a specimen. The scanning modes are inherently capable of producing three-dimensional (3-D) images due to optical sectioning and localized probing. Illumination and imaging are performed coaxially with minimal changes of optical components between modes. Multimodal images of embryos are shown to demonstrate the microscope's imaging capabilities.
植入前胚胎存活的遗传调控。
影响因子：
--
作者：
Warner,CM;Brenner,CA
通讯作者：
Brenner,CA
影响因子：
56.9
作者：
DENK, W;STRICKLER, JH;WEBB, WW
通讯作者：
WEBB, WW