Effects of water turbulence on variations in cell ultrastructure and metabolism of amino acids in the submersed macrophyte, Elodea nuttallii (Planch.) H. St. John.
Effects of water turbulence on variations in cell ultrastructure and metabolism of amino acids in the submersed macrophyte, Elodea nuttallii (Planch.) H. St. John.
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DOI：
10.1111/plb.12346
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发表时间：
2015-09
期刊：
影响因子：
3.9
通讯作者：
K. Atapaththu;A. Miyagi;K. Atsuzawa;Y. Kaneko;M. Kawai‐Yamada;T. Asaeda
中科院分区：
文献类型：
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作者：
K. Atapaththu;A. Miyagi;K. Atsuzawa;Y. Kaneko;M. Kawai‐Yamada;T. Asaeda
大型植物和水运动之间的相互作用尚未完全了解，并负责在植物细胞中的代谢和超微结构的变化，作为湍流的结果的原因在很大程度上是未知的。在本研究中，生长，代谢和超微结构的变化，在水生植物伊乐藻nuttallii，暴露于湍流30天后进行了评估。湍流是由垂直振荡的水平网格产生的。湍流降低了植物的生长，使叶细胞质化并加强了细胞壁，并且暴露于湍流的植物在茎叶绿体中积累了淀粉颗粒。淀粉颗粒的大小随着湍流的大小而增大。使用毛细管电泳-质谱法（CE-MS），代谢物组分析发现响应于湍流的代谢物积累。天冬酰胺是胁迫下植物体内主要的氨基酸，柠檬酸、抗坏血酸、草酸和γ-氨基丁酸（GABA）等有机酸也在胁迫下积累。这些结果表明，湍流对植物的生长、细胞超微结构和E. nuttallii。我们的研究结果为解释水分运动对水生植物功能的影响提供了见解。
The interactions between macrophytes and water movement are not yet fully understood, and the causes responsible for the metabolic and ultrastructural variations in plant cells as a consequence of turbulence are largely unknown. In the present study, growth, metabolism and ultrastructural changes were evaluated in the aquatic macrophyte Elodea nuttallii, after exposure to turbulence for 30 days. The turbulence was generated with a vertically oscillating horizontal grid. The turbulence reduced plant growth, plasmolysed leaf cells and strengthened cell walls, and plants exposed to turbulence accumulated starch granules in stem chloroplasts. The size of the starch granules increased with the magnitude of the turbulence. Using capillary electrophoresis-mass spectrometry (CE-MS), analysis of the metabolome found metabolite accumulation in response to the turbulence. Asparagine was the dominant amino acid that was concentrated in stressed plants, and organic acids such as citrate, ascorbate, oxalate and γ-amino butyric acid (GABA) also accumulated in response to turbulence. These results indicate that turbulence caused severe stress that affected plant growth, cell ultrastructure and some metabolic functions of E. nuttallii. Our findings offer insights to explain the effects of water movement on the functions of aquatic plants.