Keratinocytes play a role in regulating distribution patterns of recipient melanosomes in vitro
Keratinocytes play a role in regulating distribution patterns of recipient melanosomes in vitro
复制标题
DOI：
10.1046/j.0022-202x.2001.01411.x
复制
发表时间：
2001-08-01
影响因子：
6.5
通讯作者：
Boissy, RE
中科院分区：
文献类型：
--
作者：
Minwalla, L;Zhao, Y;Boissy, RE
黑皮肤角质形成细胞中的黑素体较大且单独分布，而白种人角质形成细胞中的黑素体较小且成簇分布。这种差异导致了皮肤色素沉着和光保护的差异，但对这些先天分布模式的控制却知之甚少。为了研究这一过程，使用来自不同种族背景的黑色素细胞和角质形成细胞建立共培养，并通过电镜检查。转移到角质形成细胞的黑素体分为单独的或不同的簇。还测定了单个黑素体和群集黑素体的大小。结果表明，在我们的模型系统中，来自不同种族背景的角质形成细胞中的黑素体显示出群集和单个黑素体的组合。然而，当来自深色皮肤的角质形成细胞与来自(i)深色皮肤或（ii）浅色皮肤的黑素细胞共培养时，受体黑素小体分别为(i) 77%对23%和（ii） 64%对36%。相比之下，当来自浅色皮肤的角质形成细胞与来自（iii）深色皮肤或（iv）浅色皮肤的黑素细胞共培养时，受体黑素小体分别为（iii） 34% vs 66%和（iv） 39% vs 61%。这些结果表明，无论来源如何，受体黑素体主要由来自深色皮肤的角质形成细胞单独分布，而来自浅色皮肤的角质形成细胞以膜结合簇状分布。深色或浅色供体黑色素细胞的黑素体大小也存在差异。然而，在共培养中，黑素体的大小与黑素体是单独分布还是聚集分布无关。这些结果表明，角化细胞内的调节因子决定受体黑素体的分布模式。
Melanosomes in keratinocytes of Black skin are larger and distributed individually whereas those within keratinocytes of Caucasian skin are smaller and distributed in clusters. This disparity contributes to differences in skin pigmentation and photoprotection, but the control of these innate distribution patterns is poorly understood. To investigate this process, cocultures were established using melanocytes and keratinocytes derived from different racial backgrounds and were examined by electron microscopy. Melanosomes transferred to keratinocytes were categorized as individual or in various clusters. Melanosome size was also determined for individual and clustered melanosomes. Results indicate that, in our model system, melanosomes in keratinocytes from different racial backgrounds show a combination of clustered and individual melanosomes. When keratinocytes from dark skin were cocultured with melanocytes from (i) dark skin or (ii) light skin, however, recipient melanosomes were individual versus clustered in (i) 77% vs 23% and (ii) 64% vs 36%, respectively. In contrast, when keratinocytes from light skin were cocultured with melanocytes from (iii) dark skin or (iv) light skin, recipient melanosomes were individual versus clustered in (iii) 34% vs 66% and (iv) 39% vs 61%, respectively. These results indicate that recipient melanosomes, regardless of origin, are predominantly distributed individually by keratinocytes from dark skin, and in membrane-bound clusters by those from light skin. There were also differences in melanosome size from dark or light donor melanocytes. Melanosome size was not related to whether the melanosomes were distributed individually or clustered, however, in cocultures. These results suggest that regulatory factor(s) within the keratinocyte determine recipient melanosome distribution patterns.