Albicanol antagonizes Cd-induced apoptosis through a NO/iNOS-regulated mitochondrial pathway in chicken liver cells
Albicanol antagonizes Cd-induced apoptosis through a NO/iNOS-regulated mitochondrial pathway in chicken liver cells
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Albicanol 通过 NO/iNOS 调节的鸡肝细胞线粒体途径拮抗 Cd 诱导的细胞凋亡
DOI：
10.1039/d0fo03270k
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发表时间：
2021-02-21
期刊：
影响因子：
6.1
通讯作者：
Chang, Ying
中科院分区：
文献类型：
--
作者：
Guan, Yalin;Zhao, Xia;Chang, Ying
镉（Cd）通过氧化应激诱导肝细胞损伤。Albicanol是一种从药用植物香鳞毛蕨中提取的倍半萜类化合物，以前已被证明具有抗衰老和抗氧化活性。本研究通过用CdCl_2（25 μ M）和/或albicanol（2.5 × 10 ~（-5）μ g mL ~（-1））处理鸡肝癌细胞（LMH）24 h，探讨albicanol抑制Cd诱导的LMH细胞凋亡的机制。研究结果表明，镉处理后，LMH细胞凋亡率明显增加。此外，iNOS活性和NO含量显著升高，促进了线粒体凋亡途径相关基因Bax、CytC、Caspase-3和Caspase-9的表达，抑制了该途径中Bcl-2的表达。Cd + albicanol处理组细胞凋亡率、iNOS和线粒体凋亡途径相关基因Bax、CytC、Caspase-3和Caspase-9的表达均显著降低，Bcl-2的表达显著增加。此外，分子对接支持albicanol配体和iNOS受体之间的联系。这些结果表明，albicanol可通过调节NO/iNOS介导的线粒体途径抑制镉诱导的细胞凋亡。
Cadmium (Cd) induces hepatocyte injury by oxidative stress. Albicanol is a sesquiterpenoid extracted from the medicinal plant Dryopteris fragrans that has previously been shown to exhibit anti-aging and antioxidant activity. In this study, we explored the mechanism of albicanol inhibition of the Cd-induced apoptosis of chicken hepatoma cells (LMH) by treating these cells with CdCl2 (25 mu M) and/or albicanol (2.5 x 10(-5) mu g mL(-1)) for 24 h. Under Cd treatment, the research results showed that the apoptosis rate markedly increased in LMH cells. In addition, the iNOS activity and NO content increased significantly, which promoted the expressions of genes associated with the mitochondrial apoptosis pathway (Bax, CytC, Caspase-3 and Caspase-9) and inhibited the expression of Bcl-2 in this pathway. However, Cd + albicanol co-treatment significantly reduced the apoptosis rate and the expressions of iNOS and genes associated with the mitochondrial apoptosis pathway (Bax, CytC, Caspase-3 and Caspase-9), and promoted the expression of Bcl-2 in this pathway. In addition, molecular docking supported a link between the albicanol ligand and the iNOS receptor. These results indicated that albicanol can inhibit Cd-induced apoptosis by regulating the NO/iNOS-mediated mitochondrial pathway.