Bovine mastitis associated with Prototheca blaschkeae
Bovine mastitis associated with Prototheca blaschkeae
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DOI：
10.1128/jcm.00323-08
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发表时间：
2008-06-01
影响因子：
9.4
通讯作者：
Thompson, Gertrude
中科院分区：
文献类型：
--
作者：
Marques, Sara;Silva, Eliane;Thompson, Gertrude
奶牛乳房炎是一种重要而复杂的疾病，给乳品工业造成严重的经济损失。可能涉及绿色无绿藻的生物型II菌株，最常导致与产奶量减少相关的难以治疗的慢性乳腺炎。这种类型的感染是罕见的，但世界各地报告的病例数量正在增加。为了确定葡萄牙西北部的无绿藻属乳腺炎涉及的物种种类，对41株无绿藻属菌株进行了遗传学鉴定。藻类是在6年期间收集的无绿藻分离物的一部分，从总共22个牛群中的41头奶牛的牛奶中分离，这些奶牛具有体细胞计数增加、乳房感染的轻度临床体征和对常规治疗的不成功反应的历史。对18S rDNA进行PCR扩增、扩增rDNA限制性内切酶分析和18S rDNA序列的系统发育分析。37个分离株被鉴定为佐氏毕赤酵母变种。4种为Prototheca blaschkeae。这些数据表明，高发病率的P. zopfii变种。在该地区的碳氢化合物乳腺炎，并首次证明参与P. blaschkeae与牛乳腺感染。
Bovine mastitis is an important and complex disease responsible for economic losses in the dairy industry. Biotype II strains of the green alga Prototheca zopfii can be involved, most often resulting in chronic mastitis of difficult treatment associated with reduced milk production. This type of infection is rare, but the number of reported cases is increasing worldwide. In order to determine the kind of species involved in mastitis by Prototheca in northwest Portugal, 41 Prototheca isolates were genetically characterized. The algae are part of Prototheca isolates that were collected during a 6-year period, isolated from the milk of 41 dairy cows in a total of 22 herds with a history of increasing somatic cell counts, mild clinical signs of udder infection, and unsuccessful response to the usual therapy. PCR amplification of the 18S ribosomal DNA (rDNA), amplified rDNA restriction analysis, and phylogenetic analyses of the 18S rDNA sequences were performed. Thirty-seven isolates were identified as P. zopfii var. hydrocarbonea and four as Prototheca blaschkeae. These data suggest a high incidence of P. zopfii var. hydrocarbonea mastitis in the region and demonstrate for the first time the involvement of P. blaschkeae with bovine mammary gland infections.