Gyrification of the cerebral cortex requires FGF signaling in the mammalian brain
Gyrification of the cerebral cortex requires FGF signaling in the mammalian brain
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DOI：
10.7554/elife.29285
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发表时间：
2017-11-14
期刊：
影响因子：
7.7
通讯作者：
Kawasaki, Hiroshi
中科院分区：
文献类型：
--
作者：
Matsumoto, Naoyuki;Shinmyo, Yohei;Kawasaki, Hiroshi
虽然人们认为皮质褶皱的进化是一个里程碑，允许大脑皮质中神经元数量的增加，但皮质褶皱形成的机制在很大程度上是未知的。在这里，我们显示了区域差异的成纤维细胞生长因子受体（FGFRs）的表达在发展中的雪貂大脑皮层，甚至在皮质褶皱形成。通过利用我们的雪貂子宫内电穿孔技术的优势，我们发现，皮质折叠受损的雪貂大脑皮层时，FGF信号被抑制。我们还发现，FGF信号是至关重要的生产Pax6阳性神经祖细胞的外脑室下区（OSVZ）的发展中的大脑皮层。此外，我们发现，大脑皮层的上层优先减少抑制FGF信号。我们的研究结果揭示了脑回哺乳动物大脑皮质折叠的机制。
Although it has been believed that the evolution of cortical folds was a milestone, allowing for an increase in the number of neurons in the cerebral cortex, the mechanisms underlying the formation of cortical folds are largely unknown. Here we show regional differences in the expression of fibroblast growth factor receptors (FGFRs) in the developing cerebral cortex of ferrets even before cortical folds are formed. By taking the advantage of our in utero electroporation technique for ferrets, we found that cortical folding was impaired in the ferret cerebral cortex when FGF signaling was inhibited. We also found that FGF signaling was crucial for producing Pax6-positive neural progenitors in the outer subventricular zone (OSVZ) of the developing cerebral cortex. Furthermore, we found that upper layers of the cerebral cortex were preferentially reduced by inhibiting FGF signaling. Our results shed light on the mechanisms of cortical folding in gyrencephalic mammalian brains.