MiR-26a-5p Inhibits Cell Proliferation and Enhances Doxorubicin Sensitivity in HCC Cells via Targeting AURKA
MiR-26a-5p Inhibits Cell Proliferation and Enhances Doxorubicin Sensitivity in HCC Cells via Targeting AURKA
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DOI：
10.1177/1533033819851833
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发表时间：
2019-09-30
影响因子：
2.8
通讯作者：
Li, De Yu
中科院分区：
文献类型：
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作者：
Yuan, Yan Li;Yu, HaiBo;Li, De Yu
目的：探讨miR-26 a-5 p在肝癌细胞增殖及阿霉素敏感性中的作用。方法：采用逆转录聚合酶链反应（RT-PCR）检测肝癌组织和肝癌细胞株中miR-26 a-5 p的表达。细胞计数试剂盒-8检测细胞增殖。荧光素酶报告系统检测miR-26 a-5 p与极光激酶A的关系。Western blot检测极光激酶A的表达。结果：在本研究中，我们观察到miR-26 a-5 p在肝癌组织和细胞系中表达下调。功能获得实验表明，在miR-26 a-5 p模拟物的条件下，肝癌细胞的增殖率降低。我们发现miR-26 a-5 p模拟物可以增强肝癌细胞对阿霉素的敏感性。进一步的研究表明极光激酶A是miR-26 a-5 p的靶基因。抑制极光激酶A可导致肝癌细胞增殖降低和阿霉素敏感性增加。结论：我们的研究发现miR-26 a-5 p可以通过靶向极光激酶A抑制肝癌细胞的增殖并增强其对阿霉素的敏感性。
Objective: To investigate the role of miR-26a-5p in cell proliferation and doxorubicin sensitivity in hepatocellular carcinoma. Methods: We evaluated miR-26a-5p expression in hepatocellular carcinoma tissues and cell lines by reverse transcription polymerase chain reaction. Cell Counting Kit-8 was used to examine cell proliferation. Relationship between miR-26a-5p and aurora kinase A was evaluated by luciferase report system. Western blot was used to detect expression of aurora kinase A. Results: In this study, we observed miR-26a-5p was downregulated in hepatocellular carcinoma tissues and cell lines. Gain-of-function experiments showed that proliferation rate of hepatocellular carcinoma cells decreased under condition of miR-26a-5p mimics. We found miR-26a-5p mimics could enhance doxorubicin sensitivity of hepatocellular carcinoma cells. Further study showed that aurora kinase A was target gene of miR-26a-5p. Suppression of aurora kinase A could lead to lower cell proliferation and higher doxorubicin sensitivity of hepatocellular carcinoma cells. Conclusion: Our study found that miR-26a-5p could inhibit cell proliferation and enhance doxorubicin sensitivity in hepatocellular carcinoma cells by targeting aurora kinase A.