Development of a Slow-Degrading Polymerized Curcumin Coating for Intracortical Microelectrodes
Development of a Slow-Degrading Polymerized Curcumin Coating for Intracortical Microelectrodes
复制标题
用于皮质内微电极的缓慢降解聚合姜黄素涂层的开发
DOI：
10.1021/acsabm.2c00969
复制
发表时间：
2023
影响因子：
4.7
通讯作者：
Cao, Dominica
中科院分区：
文献类型：
--
作者：
Ziemba, Alexis M.;Woodson, Mary Clare;Funnell, Jessica L.;Wich, Douglas;Balouch, Bailey;Rende, Deniz;Amato, Dahlia N.;Bao, Jonathan;Oprea, Ingrid;Cao, Dominica
皮质内微电极与脑机接口一起使用，以恢复神经系统损伤后失去的肢体功能。尽管前景看好，但皮质内微电极的记录能力会随着时间的推移而减弱，部分原因是神经炎症。由于姜黄素通过抗炎活性表现出神经保护作用，我们制备了一种300 nm厚的皮质内微电极涂层，由姜黄素和聚乙二醇(PEG)的聚氨酯共聚物组成，命名为聚(姜黄素-聚乙二醇1000氨基甲酸酯)(PCPC)。均匀的PCPC涂层将硅片的硬度降低了两个数量级，并且在几分钟内就很容易吸收水分，表明该涂层本质上是柔软和亲水的。采用体外释放模型，姜黄素从PCPC涂层洗脱到上清液中超过1周；在缓冲液中孵育8周后，大部分涂层完好无损，显示出姜黄素有可能长期释放和柔软。在活体大鼠皮质内微电极模型中评估PCPC的有效性，未包被和包被PCPC的探针在组织炎症、瘢痕形成、神经元存活和髓鞘损伤方面没有显著差异。作为第一个将无功能探针植入聚合姜黄素涂层的研究，我们已经证明了PCPC涂层的生物相容性，并提出了将聚(姜黄素)原聚合物作为皮质内电极涂层材料的设计起点。
Intracortical microelectrodes are used with brain–computer interfaces to restore lost limb function following nervous system injury. While promising, recording ability of intracortical microelectrodes diminishes over time due, in part, to neuroinflammation. As curcumin has demonstrated neuroprotection through anti-inflammatory activity, we fabricated a 300 nm-thick intracortical microelectrode coating consisting of a polyurethane copolymer of curcumin and polyethylene glycol (PEG), denoted as poly(curcumin-PEG1000carbamate) (PCPC). The uniform PCPC coating reduced silicon wafer hardness by two orders of magnitude and readily absorbed water within minutes, demonstrating that the coating is soft and hydrophilic in nature. Using an in vitro release model, curcumin eluted from the PCPC coating into the supernatant over 1 week; the majority of the coating was intact after an 8-week incubation in buffer, demonstrating potential for longer term curcumin release and softness. Assessing the efficacy of PCPC within a rat intracortical microelectrode model in vivo, there were no significant differences in tissue inflammation, scarring, neuron viability, and myelin damage between the uncoated and PCPC-coated probes. As the first study to implant nonfunctional probes with a polymerized curcumin coating, we have demonstrated the biocompatibility of a PCPC coating and presented a starting point in the design of poly(pro-curcumin) polymers as coating materials for intracortical electrodes.