Heat stress increases apical glucose transport in the chicken jejunum
Heat stress increases apical glucose transport in the chicken jejunum
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DOI：
10.1152/ajpregu.00393.2005
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发表时间：
2006-01-01
影响因子：
2.8
通讯作者：
Moretó, M
中科院分区：
文献类型：
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作者：
Garriga, C;Hunter, RR;Moretó, M
在鸡中，环境温度升高会减少食物摄入量。我们以前报道过，在热应激期间，肠粘膜吸收糖的能力增加。为了探讨温暖环境对糖摄取的影响是否是肠道对低能量摄入的适应或归因于热应激的反应，我们研究了在气候室中维持2周的肉鸡空肠顶膜和基底膜的葡萄糖转运动力学和粘膜形态。实验组为1）对照自由采食（CAL），自由采食并在热中性条件（20 ° C）下; 2）热应激自由采食（HSAL），自由采食并保持在加热环境（30 ° C）下;和3）对照成对采食（CPF），保持在热中性条件下并饲喂与HSAL组消耗的相同量的食物。CPF组和HSAL组的增重均有所降低，但只有HSAL组的血浆甲状腺激素水平低于CAL组和CPF组，而皮质酮水平高于CAL组和CPF组。空肠的鲜重和长度仅在HSAL组中减少。在HSAL鸡中，顶端钠依赖性葡萄糖转运蛋白1（SGLT-1）的活性和表达增加了约50%，而在CPF组中没有影响。在成对饲喂和加热的鸡中未观察到Kd或SGLT-1和葡萄糖转运蛋白-2 K-m的变化。这些结果支持以下观点，即肠己糖转运能力的增加完全取决于顶端SGLT-1表达对热应激的适应，而不是由于摄食量减少。
In chickens, elevated environmental temperature reduces food intake. We have previously reported that, during heat stress, the intestinal mucosa has an increased capacity to take up sugars. To investigate whether the effects of warm environment on sugar uptake are an intestinal adaptation to lower energy intake or a response attributable to heat stress, we examined the glucose transport kinetics of apical and basolateral membranes of the jejunum and the mucosal morphology of broiler chickens maintained in climatic chambers for 2 wk. Experimental groups were 1) control ad libitum (CAL), fed ad libitum and in thermoneutral conditions ( 20 degrees C); 2) heat stress ad libitum ( HSAL), fed ad libitum and kept in a heated environment ( 30 degrees C); and 3) control pair-fed (CPF), maintained in thermoneutral conditions and fed the same amount of food as that consumed by the HSAL group. Both the CPF and the HSAL groups showed reduced body weight gain, but only the HSAL chickens had lower plasma thyroid hormones and higher corticosterone than CAL and CPF groups. The fresh weight and length of the jejunum were only reduced in the HSAL group. The activity and expression of apical sodium-dependent glucose transporter 1 (SGLT-1) were increased by similar to 50% in the HSAL chickens, without effects in the CPF group. No changes in K-d or in SGLT-1 and glucose transporter-2 K-m were observed in the pair-fed and heated birds. These results support the view that increased intestinal hexose transport capacity is entirely dependent on adaptations of apical SGLT-1 expression to heat stress and is not due to reduced food intake.