Expression of metastasis-associated protein 1 (MTA1) in benign endometrium and endometrial adenocarcinomas
Expression of metastasis-associated protein 1 (MTA1) in benign endometrium and endometrial adenocarcinomas
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DOI：
10.1016/j.humpath.2006.01.024
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发表时间：
2006-06-01
期刊：
影响因子：
3.3
通讯作者：
Kumar, Rakesh
中科院分区：
文献类型：
--
作者：
Balasenthil, Seetharaman;Broaddus, Russell R.;Kumar, Rakesh
子宫内膜癌是女性生殖道最常见的恶性肿瘤之一。转移相关蛋白1(MTA1)是Mi-2/核小体重构和去乙酰化复合体的组成部分，是乳腺癌细胞中雌激素受体的有效辅阻遏子。MTA1在多种肿瘤中的表达已被证实，但在子宫内膜癌中从未被研究过。我们采用免疫组织化学和Western blotting方法研究了MTA1在良性子宫内膜不同阶段以及子宫内膜样腺癌中的表达。在增殖期和分泌期，MTA1在腺室和间质均有表达，定位于胞核和胞浆。与增殖期相比，分泌期MTA1的表达较弱。在绝经后切片中，MTA1染色见于腺体和间质，定位于胞核和胞浆。对6例肿瘤标本进行免疫印迹分析，结果显示所有肿瘤组织中MTA1的表达均升高。对70例不同级别子宫内膜样腺癌的肿瘤芯片进行免疫组织化学染色，结果显示有53例(75.7%)MTA1表达增强。在1级和2级肿瘤中，MTA1在胞核和胞浆均有表达。有趣的是，在3级肿瘤中，MTA1仅定位于细胞质。我们的结果提示MTA1在子宫内膜癌中有潜在的作用。(C)2006 Elsevier Inc.保留所有权利。
Endometrial carcinoma is one of the most common malignancies of the female genital tract. Metastasis-associated protein 1 (MTA1) is a component of the Mi-2/nucleosome remodeling and deacetylating complex and acts as a potent corepressor of estrogen receptor in breast cancer cells. MTA1 expression has been demonstrated in various cancers but has never been explored in endometrial carcinoma. We investigated the expression profile of MTA1 in different stages of benign endometrium as well as in endometrial endometrioid adenocarcinoma using immunohistochemistry and Western blotting. In the proliferative and secretory phases, MTA1 was expressed in both the glandular and the stromal compartments and was localized in nucleus and cytoplasm of these cells. MTA1 expression in secretory phase was less prominent when compared with the proliferative phase. In postmenopausal sections, MTA1 staining was observed in both glandular and stromal compartments and was localized in both nucleus and cytoplasm. Western blot analysis of 6 tumor specimens showed increased expression of MTA1 in all the tumors analyzed. Immunohistochemical staining performed on tumor microarray containing 70 endometrial endometrioid adenocarcinomas of various grades showed increased expression of MTA1 in 53 (75.7%) tumors. In grade 1 and grade 2 tumors, MTA1 was present in both nucleus and cytoplasm. Interestingly, in grade 3 tumors, MTA1 was localized in the cytoplasm only. Our results suggest a potential role of MTA1 in endometrial carcinomas. (c) 2006 Elsevier Inc. All rights reserved.