ApoE isoform does not influence skeletal muscle regeneration in adult mice.
ApoE isoform does not influence skeletal muscle regeneration in adult mice.
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DOI：
10.3389/fphys.2023.1302695
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发表时间：
2023
影响因子：
4
通讯作者：
中科院分区：
文献类型：
--
作者：
引言：载脂蛋白E（ApoE）已被证明是必要的适当的骨骼肌再生。与这一发现一致，骨骼肌干细胞（MuSC）的单细胞RNA测序分析显示，Apoe是激活后下调的静止MuSC的最高标志物。本研究的目的是确定肌肉再生是否在携带三种常见的人类ApoE同种型（称为ApoE 2，E3和E4）之一的小鼠中发生改变。 研究方法：采用组织形态计量学分析来评估ApoE 2、E3和E4小鼠从氯化钡诱导的体内肌肉损伤恢复14天后的肌肉再生，并分离原代MuSC以评估ApoE 2、E3和E4 MuSC的体外增殖和分化。 结果如下：通过截面积、肌纤维横截面积（CSA）、肌纤维和MuSC丰度/纤维评价，ApoE亚型的基础骨骼肌表型无差异。虽然在比目鱼肌中的纤维型频率没有差异，但与ApoE 3相比，ApoE 4小鼠跖肌中的IIa型相对频率显著较低。此外，ApoE亚型不影响肌肉再生评估的纤维频率，纤维CSA，mycoplasma和MuSC丰度。最后，在任何ApoE亚型之间MuSC的增殖能力或肌源性分化潜能没有差异。 讨论内容：总的来说，这些数据表明ApoE同种型对骨骼肌损伤后再生能力或体外MuSC表型的标称影响。
Introduction: Apolipoprotein E (ApoE) has been shown to be necessary for proper skeletal muscle regeneration. Consistent with this finding, single-cell RNA-sequencing analyses of skeletal muscle stem cells (MuSCs) revealed that Apoe is a top marker of quiescent MuSCs that is downregulated upon activation. The purpose of this study was to determine if muscle regeneration is altered in mice which harbor one of the three common human ApoE isoforms, referred to as ApoE2, E3 and E4. Methods: Histomorphometric analyses were employed to assess muscle regeneration in ApoE2, E3, and E4 mice after 14 days of recovery from barium chloride-induced muscle damage in vivo, and primary MuSCs were isolated to assess proliferation and differentiation of ApoE2, E3, and E4 MuSCs in vitro. Results: There was no difference in the basal skeletal muscle phenotype of ApoE isoforms as evaluated by section area, myofiber cross-sectional area (CSA), and myonuclear and MuSC abundance per fiber. Although there were no differences in fiber-type frequency in the soleus, Type IIa relative frequency was significantly lower in plantaris muscles of ApoE4 mice compared to ApoE3. Moreover, ApoE isoform did not influence muscle regeneration as assessed by fiber frequency, fiber CSA, and myonuclear and MuSC abundance. Finally, there were no differences in the proliferative capacity or myogenic differentiation potential of MuSCs between any ApoE isoform. Discussion: Collectively, these data indicate nominal effects of ApoE isoform on the ability of skeletal muscle to regenerate following injury or the in vitro MuSC phenotype.
影响因子：
4
作者：
Nalbandian M;Radak Z;Takeda M
通讯作者：
Takeda M