Highly parallel measurements of interaction kinetic constants with a microfabricated optomechanical device
Highly parallel measurements of interaction kinetic constants with a microfabricated optomechanical device
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DOI：
10.1063/1.3211382
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发表时间：
2009-08-17
影响因子：
4
通讯作者：
Quake, Stephen R.
中科院分区：
文献类型：
--
作者：
Bates, Steven R.;Quake, Stephen R.
我们使用分子相互作用的机械捕获来展示一种高度并行的方法来测量生物分子相互作用的动力学。这种方法每次测量消耗25 fmol的材料，并且允许在单个实验中进行320次测量。我们测量了6-His和T7表位标签与其抗体的相互作用的缔合和解离曲线，从中我们确定了解离速率、结合速率和解离常数。
We used mechanical trapping of molecular interactions to demonstrate a highly parallel approach to measure the kinetics of biomolecular interactions. This approach consumes 25 fmol of material per measurement and permits 320 measurements in a single experiment. We measured association and dissociation curves for the interactions of 6-His and T7 epitope tags with their antibodies, from which we determined the off rates, on rates, and dissociation constants.