Amphipathic DNA Origami Nanoparticles to Scaffold and Deform Lipid Membrane Vesicles
Amphipathic DNA Origami Nanoparticles to Scaffold and Deform Lipid Membrane Vesicles
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DOI：
10.1002/anie.201501173
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发表时间：
2015-05-26
影响因子：
16.6
通讯作者：
Schwille, Petra
中科院分区：
文献类型：
--
作者：
Czogalla, Aleksander;Kauert, Dominik J.;Schwille, Petra
我们报告了一种合成生物学启发的方法，用于工程化两亲性DNA折纸结构作为膜支架工具。该结构具有用胆固醇衍生的锚修饰的平坦的膜结合界面。在其侧面上的粘性寡核苷酸突出端能够实现横向相互作用，导致在膜上形成有序阵列。这种紧密和规则的排列使我们的DNA折纸能够使独立的脂质膜变形，模仿形成涂层的蛋白质的生物活性，例如来自I-/F-BAR家族的蛋白质。
We report a synthetic biology-inspired approach for the engineering of amphipathic DNA origami structures as membrane-scaffolding tools. The structures have a flat membrane-binding interface decorated with cholesterol-derived anchors. Sticky oligonucleotide overhangs on their side facets enable lateral interactions leading to the formation of ordered arrays on the membrane. Such a tight and regular arrangement makes our DNA origami capable of deforming free-standing lipid membranes, mimicking the biological activity of coat-forming proteins, for example, from the I-/F-BAR family.