COMPLETE AMINO-ACID-SEQUENCE OF THE HUMAN PROGESTERONE-RECEPTOR DEDUCED FROM CLONED CDNA
COMPLETE AMINO-ACID-SEQUENCE OF THE HUMAN PROGESTERONE-RECEPTOR DEDUCED FROM CLONED CDNA
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DOI：
10.1016/0006-291x(87)91416-1
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发表时间：
1987-03-13
影响因子：
3.1
通讯作者：
MILGROM, E
中科院分区：
文献类型：
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作者：
MISRAHI, M;ATGER, M;MILGROM, E
构建含有与来自人乳腺癌T47-D细胞的信使RNA互补的DNA的cDNA gt 10文库，并用编码兔孕酮受体的cDNA探针筛选。四个重叠克隆已测序。开放阅读框对应于分子量为98，868 Da的933个氨基酸的蛋白质。被认为参与DNA结合的富含半胱氨酸的碱性区域在人和兔受体中是完全同源的，而被认为发生激素结合的C-末端仅相差一个氨基酸变化。与兔受体一样，人孕酮受体的特征在于其N-末端区域的脯氨酸含量非常高。当印迹来自人乳腺癌细胞系T47-D和MCF-7或来自正常人子宫组织的mRNA并用克隆的cDNA探测时，观察到四个主要条带（5100、4300、3700和2900个核苷酸）。
A .lambda.gt10 library containing DNAs complementary to messenger RNAs from human breast cancer T47-D cells was constructed and screened with a cDNA probe encoding the rabbit progesterone receptor. Four overlapping clones have been sequenced. The open reading frame corresponds to a protein of 933 amino acids with a molecular weight of 98,868 Da. The cysteine rich basic region supposed to be involved in DNA binding is completely homologous in the human and rabbit receptors, whereas the C-terminal end, where hormone binding is thought to take place, differs by a single amino acid change. The human progesterone receptor is characterized, as is the rabbit receptor, by the very high proline content of its N-terminal region. When mRNAs from either human breast cancer cell lines T47-D and MCF-7 or from normal human uterus tissue were blotted and probed with the cloned cDNA, four main bands were observed (5100, 4300, 3700, and 2900 nucleotides).