PDGF-AA and bFGF mediate B104CM-induced proliferation of oligodendrocyte precursor cells
PDGF-AA and bFGF mediate B104CM-induced proliferation of oligodendrocyte precursor cells
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PDGF-AA和bFGF介导B104CM诱导的少突胶质细胞前体细胞增殖
DOI：
10.3892/ijmm.2012.1110
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发表时间：
2012-11-01
影响因子：
5.4
通讯作者：
Lu, He-Zuo
中科院分区：
文献类型：
--
作者：
Hu, Jian-Guo;Wu, Xing-Jun;Lu, He-Zuo
B104神经母细胞瘤细胞（B104 CM）的条件培养基在体外诱导少突胶质前体细胞（OPCs）增殖，表明B104 CM中含有的某些因子提供指导OPCs增殖的指令信号。然而，OPC增殖因子存在于B104 CM尚未确定。血小板衍生生长因子AA（PDGF-AA）、碱性成纤维细胞生长因子（bFGF）和胰岛素样生长因子-1（IGF-1）已被报道作为OPC增殖的有效促分裂剂。这增加了B104 CM通过分泌PDGF-AA、bFGF和/或IGF-1诱导OPC增殖的可能性。本研究检测了B104细胞和B104 CM中PDGF-AA、bFGF和IGF-1的表达水平，并观察了其受体在OPCs中的表达。结果表明，这些生长因子在B104细胞和B104 CM中均有表达。所有3种受体，PDGFR，FGFR 2和IGF-1 R，也在OPCs中检测到。此外，AG 1295（PDGFR抑制剂）和PD 173074（FGFR抑制剂）均可显着降低B104 CM刺激的OPC增殖。然而，用AG 1204抑制IGF-1 R并不影响OPCs的增殖。我们的研究表明，B104 CM中的PDGF-AA和bFGF是刺激OPC增殖的两个关键因素。
The conditioned medium from B104 neuroblastoma cells (B104CM) induces proliferation of oligodendrocyte precursor cells (OPCs) in vitro, which indicates that certain factors contained within B104CM give instructional signals that direct the proliferation of OPCs. However, the OPC-proliferative factors present in B104CM have yet to be identified. Platelet-derived growth factor AA (PDGF-AA), basic fibroblast growth factor (bFGF) and insulin-like growth factor-1 (IGF-1) have been reported to act as potent mitogens for OPC proliferation. This raises the possibility that B104CM induces proliferation of OPCs through secretion of PDGF-AA, bFGF and/or IGF-1. In the present study, we detected the expression and levels of PDGF-AA, bFGF and IGF-1 in B104 cells and B104CM, and observed the expression of their receptors in OPCs. The results indicated that these growth factors were expressed in B104 cells and B104CM. All 3 receptors, PDGFR, FGFR2 and IGF-1R, were also detected in OPCs. Furthermore, B104CM-stimulated OPC proliferation could be markedly decreased by both AG1295 (an inhibitor of PDGFR) and PD173074 (an inhibitor of FGFR). However, the inhibition of IGF-1R with AG1204 did not affect the proliferation of OPCs. Our study suggests that the PDGF-AA and bFGF in B104CM are 2 key factors that stimulate OPC proliferation.