Effect of prolonged exposure to low concentrations of formaldehyde on the corticotropin releasing hormone neurons in the hypothalamus and adrenocorticotropic hormone cells in the pituitary gland in female mice
Effect of prolonged exposure to low concentrations of formaldehyde on the corticotropin releasing hormone neurons in the hypothalamus and adrenocorticotropic hormone cells in the pituitary gland in female mice
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DOI：
10.1016/j.brainres.2004.03.070
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发表时间：
2004-07-02
期刊：
影响因子：
2.9
通讯作者：
Sasaki, F
中科院分区：
文献类型：
--
作者：
Sari, DK;Kuwahara, S;Sasaki, F
我们研究的影响下丘脑-垂体-肾上腺（HPA）轴长期暴露于低水平的甲醛在雌性C3 H/He小鼠，使用免疫细胞化学和RT-PCR方法。将两组雌性小鼠暴露于不同浓度（0、80、400、2000 ppb）的甲醛吸入，16 h/d，5 d/周，持续12周。然后检查下丘脑中的促肾上腺皮质激素释放激素（CRH）免疫反应（IR）神经元，以及垂体中的促肾上腺皮质激素（ACTH）免疫反应细胞和ACTH mRNA。一组包括假手术对照小鼠。另一组在接触甲醛之前通过注射卵清蛋白（OVA）和明矾使其过敏，因为大多数病态建筑综合症（SBS）患者都是患有过敏性疾病的女性。在暴露期间，这些动物进一步暴露于雾化OVA作为加强剂四次。我们的研究结果表明，在非过敏（NAG）组CRH-IR神经元的数量呈剂量依赖性增加。在ACTH ir细胞和ACTH mRNA中发现了类似的模式。过敏模型组基础HPA活性指标均升高。此外，AG小鼠似乎对最低浓度的甲醛有反应，在最高浓度的甲醛下，HPA活性的所有指标都降低。这些结果涉及到一个重要的临床问题，也有更广泛的HPA监管领域的影响。我们的结论是，我们的实验系统可能是一个合适的动物模型SBS和/或多化学敏感性（MCS）。(C)2004 Elsevier B. V.保留所有权利。
We examine the effect on the hypothalamus-pituitary-adrenal gland (HPA) axis of prolonged exposure to low levels of formaldehyde in female C3H/He mice, using immunocytochemical and RT-PCR methods. Two groups of female mice were exposed to differing concentrations (0, 80, 400, 2000 ppb) of formaldehyde inhalation for 16 h/day, 5 days/week, for 12 weeks. The corticotropin releasing hormone (CRH)-immunoreactive (ir) neurons in the hypothalamus were then examined, together with the adrenocorticotropin hormone (ACTH)-ir cells and ACTH rnRNA in the pituitary. One group comprised sham control mice. The other group was made allergic by injection of ovalbumin (OVA) and alum prior to exposure to formaldehyde, since most sick building syndrome (SBS) sufferers are women with allergic disease. These animals were further exposed to aerosolized OVA as a booster four times during the exposure period. Our results showed a dose-dependent increase in the number of CRH-ir neurons in the non-allergy (NAG) group. A similar pattern was found in ACTHir cells and ACTH mRNA. The allergy (AG) model group showed an increase in basal levels of all markers of HPA activity. Moreover, the AG mice appeared to respond to the lowest concentration of formaldehyde, and all indices of HPA activity were reduced at the highest concentrations of formaldehyde. These results relate to an important clinical issue and also have implications in the broader area of HPA regulation. We conclude that our experimental system may be a suitable animal model for SBS and/or multiple chemical sensitivity (MCS). (C) 2004 Elsevier B.V. All rights reserved.