Nuclear export of influenza virus ribonucleoproteins: Identification of an export intermediate at the nuclear periphery
Nuclear export of influenza virus ribonucleoproteins: Identification of an export intermediate at the nuclear periphery
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DOI：
10.1006/viro.2001.0833
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发表时间：
2001-04-10
期刊：
影响因子：
3.7
通讯作者：
Whittaker, GR
中科院分区：
文献类型：
--
作者：
Ma, K;Roy, AMM;Whittaker, GR
流感病毒生命周期的一个关键阶段是基因组核糖核蛋白（vRNP）从核内部，穿过核膜，并进入细胞质的调节易位。两种病毒蛋白质，M1和NS2，以前被认为是vRNP输出的介质。我们在这里表明，vRNP核输出是由来普霉素B（LMB），细胞因子CRM 1的抑制剂。在LM α处理的细胞中，vRNP被发现在定位于核纤层的外周核位置中。vRNP不与M1或NS2共定位。在感染后期的细胞原位提取也揭示了核vRNP的外周定位，而在感染早期vRNP分散在整个核内部。我们认为，在核周边的vRNP代表流感病毒核输出途径中的一种新的中间体。(C)北京：清华大学出版社.
A critical phase of the influenza virus life cycle is the regulated translocation of genomic ribonucleoproteins (vRNPs) from the nuclear interior, across the nuclear envelope, and into the cytoplasm. Two viral proteins, M1 and NS2, have previously been implicated as mediators of vRNP export. We show here that vRNP nuclear export is prevented by leptomycin B (LMB), an inhibitor of the cellular factor CRM1. in LM a-treated cells, vRNPs were found in a peripheral nuclear location that localized with the nuclear lamina. vRNPs were not colocalized with either M1 or NS2. In situ extraction of cells late in infection also revealed a peripheral localization of nuclear vRNPs, whereas early in infection vRNPs were dispersed throughout the nuclear interior. We believe that vRNPs at the nuclear periphery represent a novel intermediate in the influenza Virus nuclear export pathway. (C) 2001AcademicPress.