INTERACTION OF A LIVER-SPECIFIC NUCLEAR FACTOR WITH THE FIBRINOGEN AND ALPHA-1-ANTITRYPSIN PROMOTERS
INTERACTION OF A LIVER-SPECIFIC NUCLEAR FACTOR WITH THE FIBRINOGEN AND ALPHA-1-ANTITRYPSIN PROMOTERS
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DOI：
10.1126/science.3499668
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发表时间：
1987-10-30
期刊：
影响因子：
56.9
通讯作者：
CRABTREE, GR
中科院分区：
文献类型：
--
作者：
COURTOIS, G;MORGAN, JG;CRABTREE, GR
发育过程中基因的有序和顺序激活被认为与主要在转录水平上激活的一组调控蛋白的选择性表达有关。在肝细胞中发现了一种核蛋白，但其他细胞类型没有发现，它与肝细胞特异性转录β基因所需的序列结合。纤维蛋白原链。这种蛋白质，肝细胞核因子1(HNF1)，也与α基因的最佳启动子功能所需的同源序列相互作用。纤维蛋白原链和α1-抗胰蛋白酶。几个不在肝脏表达的病毒和细胞基因的启动子或增强子区域不竞争这种结合。HNF1的限制性表达及其与几个肝脏特异基因控制区的选择性相互作用表明，它参与了肝脏发育调控基因的表达。
The orderly and sequential activation of genes during development is hypothesized to be related to the selective expression of groups of regulatory proteins activing primarily at the level of transcription. A nuclear-protein was found in hepatocytes, but not other cell types, that binds to a sequence required for hepatocyte-specific transcription of the gene for the .beta. chain of fibrinogen. This protein, hepatocyte nuclear factor 1 (HNF1), also interacts with homologous sequences required for optimal promoter function of the genes for the .alpha. chain of fibrinogen and .alpha.1-antitrypsin. The promoter or enhancer regions for several viral and cellular genes not expressed in the liver did not compete for this binding. The restricted expression of HNF1 and its selective interaction with the control regions of several liver-specific genes indicate that it is involved in developmentally regulated gene expression in the liver.