Nanoarchitecturing of Natural Melanin Nanospheres by Layer-by-Layer Assembly: Macroscale Anti-inflammatory Conductive Coatings with Optoelectronic Tunability
Nanoarchitecturing of Natural Melanin Nanospheres by Layer-by-Layer Assembly: Macroscale Anti-inflammatory Conductive Coatings with Optoelectronic Tunability
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DOI：
10.1021/acs.biomac.7b00336
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发表时间：
2017-06-01
影响因子：
6.2
通讯作者：
Shim, Bong Sup
中科院分区：
文献类型：
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作者：
Eom, Taesik;Woo, Kyungbae;Shim, Bong Sup
天然黑色素是具有多种功能的生物相容性导体。在这里，我们报告了使用直接从棕褐色墨水中提取的黑色素纳米颗粒（MNP）通过逐层（LBL）组装来制造多功能聚（乙烯醇）/黑色素纳米复合材料。 LBL 组件可以轻松操纵纳米纹理，并通过改变溶剂质量来控制宏观薄膜的纳米厚度。在此过程中监测时间分辨吸收，并通过分形维数和空隙度分析进行定量研究。纳米结构的能力提供了完整单层形成的确认，并导致 MNP 薄膜的可调虹彩反射颜色。此外，MNP 薄膜具有持久的电化学导电性，1000 次循环的电荷存储能力增强证明了这一点。此外，与裸露的 ITO 相比，覆盖 ITO（氧化铟锡）基质的 MNP 显着减少了原始 264.7 巨噬细胞的炎症细胞因子 TNF-α 的分泌，在有和没有脂多糖内毒素的情况下分别减少了 5 倍和 1.8 倍。这些结果凸显了 MNP LBL 薄膜的光电器件级可调性以及抗炎生物相容性。这种性能的结合应该使这些薄膜对于生物电子设备应用特别有趣，例如电子药物、人造仿生器官、生物传感器和可植入设备。
Natural melanins are biocompatible conductors with versatile functionalities. Here, we report fabrication of multifunctional poly(vinyl alcohol)/melanin nanocomposites by layer-by-layer (LBL) assembly using melanin nanoparticles (MNPs) directly extracted from sepia officinalis inks. The LBL assembly offers facile manipulation of nanotextures as well as nm-thickness control of the macroscale film by varying solvent qualities. The time-resolved absorption was monitored during the process and quantitatively studied by fractal dimension and lacunarity analysis. The capability of nanoarchitecturing provides confirmation of complete monolayer formation and leads to tunable iridescent reflective colors of the MNP films. In addition, the MNP films have durable electrochemical conductivities as evidenced by enhanced charge storage capacities for 1000 cycles. Moreover, the MNP covered ITO (indium tin oxide) substrates significantly reduced secretion of inflammatory cytokines, TNF-alpha, by raw 264.7 macrophage cells compared to bare ITO, by a factor of 5 and 1.8 with and without lipopolysaccharide endotoxins, respectively. These results highlight the optoelectronic device-level tunability along with the anti-inflammatory biocompatibility of the MNP LBL film. This combination of performance should make these films particularly interesting for bioelectronic device applications such as electroceuticals, artificial bionic organs, biosensors, and implantable devices.