Change in N-Glycosylation of Plasma Proteins in Japanese Semisupercentenarians.
Change in N-Glycosylation of Plasma Proteins in Japanese Semisupercentenarians.
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DOI：
10.1371/journal.pone.0142645
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
SONIC (Septuagenarians, Octogenarians, Nonagenarians Investigation with Centenarians)
中科院分区：
文献类型：
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作者：
Miura Y;Hashii N;Tsumoto H;Takakura D;Ohta Y;Abe Y;Arai Y;Kawasaki N;Hirose N;Endo T;SONIC (Septuagenarians, Octogenarians, Nonagenarians Investigation with Centenarians)
使用石墨化碳柱，通过液相色谱/质谱法（LC/MS）对日本半超百岁老人（SSC）（平均106.7岁）、老年对照组（平均71.6岁）和年轻对照组（平均30.2岁）的血浆蛋白进行N-糖组学分析。使用多变量分析鉴别SSC中的特征性N-聚糖;正交投影到潜在结构（O-PLS）。获得的结果表明，SSC中多分支和高度唾液酸化的N-聚糖以及无乳和/或二等分N-聚糖增加，而双触角N-聚糖减少。由于多分支和高度唾液酸化的N-聚糖与抗炎活性有关，因此这些变化可能在SSC中观察到的增强的慢性炎症中发挥作用。SSC中炎症蛋白如CRP、脂联素、IL-6和TNF-α水平升高。这些结果表明，对炎症的反应可能在人类的长寿和健康衰老中发挥重要作用。这是第一项研究表明，血浆蛋白的N-聚糖与人类的极端长寿和健康衰老有关。
An N-glycomic analysis of plasma proteins was performed in Japanese semisupercentenarians (SSCs) (mean 106.7 years), aged controls (mean 71.6 years), and young controls (mean 30.2 years) by liquid chromatography/mass spectrometry (LC/MS) using a graphitized carbon column. Characteristic N-glycans in SSCs were discriminated using a multivariate analysis; orthogonal projections to latent structures (O-PLS). The results obtained showed that multi-branched and highly sialylated N-glycans as well as agalacto- and/or bisecting N-glycans were increased in SSCs, while biantennary N-glycans were decreased. Since multi-branched and highly sialylated N-glycans have been implicated in anti-inflammatory activities, these changes may play a role in the enhanced chronic inflammation observed in SSCs. The levels of inflammatory proteins, such as CRP, adiponectin, IL-6, and TNF-α, were elevated in SSCs. These results suggested that responses to inflammation may play an important role in extreme longevity and healthy aging in humans. This is the first study to show that the N-glycans of plasma proteins were associated with extreme longevity and healthy aging in humans.