Instant Pot for antigen retrieval: a simple, safe and economical method for use in immunohistochemistry.
Instant Pot for antigen retrieval: a simple, safe and economical method for use in immunohistochemistry.
复制标题
DOI：
10.2144/btn-2022-0043
复制
发表时间：
2023-05
期刊：
影响因子：
2.7
通讯作者：
中科院分区：
文献类型：
--
作者：
在这里，作者报告了一种简单的方法，使用常用的商业Instant Pot®进行免疫组织化学抗原修复。它提供了一种经过验证的替代以前的抗原修复方法，采用水浴，微波炉或科学级压力锅。速溶锅可以设置为获得各种所需的温度，并且使用简单，使其非常适合优化。即时锅法是一种简单，安全和廉价的替代手段进行福尔马林固定石蜡包埋切片免疫组织化学。已使用几种不同的单克隆抗体（包括针对细胞表面或细胞内抗原的单克隆抗体）对其进行了验证。因此，它应该是有用的各种研究实验室以及本科实验室课程。描述了一种使用低成本、容易获得的Instant Pot®进行热介导抗原修复的经济方法。这种方法易于控制，安全，可由各个研究实验室可靠地使用。使用Instant Pot®“烹饪”：一种易于控制、安全、廉价且灵活的方法，用于进行免疫组织化学的热诱导抗原修复。
Here, the authors report a simple method to perform antigen retrieval using a commonly available commercial Instant Pot® for immunohistochemistry. It provides a validated alternative to previous antigen retrieval methods that employ water baths, microwave ovens or scientific-grade pressure cookers. The Instant Pot can be set to obtain a variety of desired temperatures and is straightforward to use, making it extremely amenable to optimization. The Instant Pot method is an easy, safe and inexpensive alternative means to perform immunohistochemistry on formalin-fixed paraffin-embedded sections. It has been validated using several different monoclonal antibodies including ones directed against cell surface or intracellular antigens. As a result, it should be useful for a variety of research labs as well as undergraduate laboratory courses. An economical method of heat-mediated antigen retrieval using a low-cost, readily available Instant Pot® is described. This approach is easily controlled, safe and can be reliably used by individual research labs. “Cooking” with Instant Pot®: an easily controlled, safe, inexpensive and flexible method for performing heat-induced antigen retrieval for immunohistochemistry.