Omega-3 supplementation can restore glutathione levels and prevent oxidative damage caused by prenatal ethanol exposure
Omega-3 supplementation can restore glutathione levels and prevent oxidative damage caused by prenatal ethanol exposure
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DOI：
10.1016/j.jnutbio.2012.04.003
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发表时间：
2013-05-01
影响因子：
5.6
通讯作者：
Christie, Brian R.
中科院分区：
文献类型：
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作者：
Patten, Anna R.;Brocardo, Patricia S.;Christie, Brian R.
产前乙醇暴露（PNEE）会导致大脑结构和功能的长期缺陷。在这项研究中，我们研究了 PNEE 对成人大脑抗氧化能力和氧化应激的影响，特别关注已知受乙醇影响的四个大脑区域：小脑、前额叶皮层和海马体（阿蒙角和齿状回亚区）。我们利用了胎儿酒精谱系障碍的流质饮食模型，该模型在整个妊娠期间提供给怀孕的斯普拉格-道利大鼠。为了检查补充 omega-3 脂肪酸的治疗潜力，一组动物从出生到成年都接受富含 omega-3 的饮食，以检查这些脂肪酸是否可以改善由于 PNEE 导致的抗氧化能力缺陷。我们的结果表明，PNEE 导致所分析的所有四个大脑区域的谷胱甘肽水平长期下降，并伴随着脂质过氧化（氧化损伤的标志）的增加。这些结果表明，PNEE 会引起大脑抗氧化能力的长期变化，这可能导致氧化应激状态。产后补充 omega-3 能够增加 PNEE 动物的谷胱甘肽水平并减少脂质过氧化，部分逆转酒精暴露的影响，特别是在齿状回和小脑中。这是第一项研究表明补充 omega-3 对 PNEE 具有有益作用，可减少氧化应激并增强抗氧化能力。 (C) 2013 Elsevier Inc. 保留所有权利。
Prenatal ethanol exposure (PNEE) causes long-lasting deficits in brain structure and function. In this study, we have examined the effect of PNEE on antioxidant capacity and oxidative stress in the adult brain with particular focus on four brain regions known to be affected by ethanol: cerebellum, prefrontal cortex and hippocampus (cornu ammonis and dentate gyrus subregions). We have utilized a liquid diet model of fetal alcohol spectrum disorders that is supplied to pregnant Sprague-Dawley rats throughout gestation. To examine the therapeutic potential of omega-3 fatty acid supplementation, a subset of animals were provided with an omega-3-enriched diet from birth until adulthood to examine whether these fatty acids could ameliorate any deficits in antioxidant capacity that occurred due to PNEE. Our results showed that PNEE caused a long-lasting decrease in glutathione levels in all four brain regions analyzed that was accompanied by an increase in lipid peroxidation, a marker of oxidative damage. These results indicate that PNEE induces long-lasting changes in the antioxidant capacity of the brain, and this can lead to a state of oxidative stress. Postnatal omega-3 supplementation was able to increase glutathione levels and reduce lipid peroxidation in PNEE animals, partially reversing the effects of alcohol exposure, particularly in the dentate gyrus and the cerebellum. This is the first study where omega-3 supplementation has been shown to have a beneficial effect in PNEE, reducing oxidative stress and enhancing antioxidant capacity. (C) 2013 Elsevier Inc. All rights reserved.