Conservation of msp, the gene encoding the major outer membrane protein of oral Treponema spp
Conservation of msp, the gene encoding the major outer membrane protein of oral Treponema spp
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DOI：
10.1128/jb.179.4.1082-1089.1997
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发表时间：
1997-02-01
影响因子：
3.2
通讯作者：
McBride, BC
中科院分区：
文献类型：
--
作者：
Fenno, JC;Wong, GWK;McBride, BC
龋密螺旋体的主要表面蛋白（Msp）被认为是牙周病中螺旋体与牙龈上皮相互作用的中介，先前的研究表明龋密螺旋体ATCC 35405的Msp具有孔蛋白活性，能去极化上皮细胞膜，与细胞外基质结合；编码ATCC 35405的Msp基因最近被克隆、测序并在大肠杆菌中表达（J. C. Fenno， K.-H.）。李建军，李建军，李建军，等。微生物学通报。2008(3):389 - 396。在本研究中，我们在几种口腔螺旋体中鉴定了编码msp样蛋白的基因。针对ATCC 35405 Msp的抗体与齿状螺旋体的Msp蛋白ATCC 35404和T. vincii反应强烈，与齿状螺旋体的ATCC 33520反应非常弱，而与齿状螺旋体OTK、T. socranskii和T. pectinovorum不反应。利用ATCC 35405 DNA序列bank msp引物，采用PCR方法对denticola菌株和vinincentii菌株的msp位点进行了鉴定，Southern blot分析显示至少有3组相关的msp DNA序列。msp基因5′和3′端DNA序列比较显示，msp基因侧翼区和信号肽编码区同源性较高，而成熟肽编码区同源性低至50%。测定了齿状霉ATCC 33520和OTK的全部msp DNA序列，并将推导出的msp氨基酸序列与先前报道的ATCC 35405的msp序列进行了比较，结果表明msp位点在口腔梅毒体中是保守的，但不同菌株的成熟msp肽之间存在显著差异，在此基础上进一步研究了msp蛋白的抗原结构域、功能结构域和物理结构。将加强对Msp在口腔螺旋体相关细胞病理学中的作用的理解。
The major surface protein (Msp) of Treponema denticola has been implicated as a mediator of the interaction between the spirochete and the gingival epithelium in periodontal diseases, Previous studies showed that the Msp of T. denticola ATCC 35405 had porin activity, depolarized epithelial cell membranes, bound to extracellular matric; components of epithelial cells, and formed a regular hexagonal surface array in the treponemal outer membrane, The gene encoding Msp in ATCC 35405 was recently cloned, sequenced, and expressed in Escherichia coli (J. C. Fenno, K.-H. Muller, and B. C. McBride, J. Bacteriol. 178:2489-2496, 1996). In the present study, we identified genes encoding Msp-like proteins in several oral spirochetes. A prominent heat-modifiable Msp-like protein having an apparent molecular mass of between 43 and 64 kDa was present in all oral spirochete strains tested, Antibodies raised against the ATCC 35405 Msp reacted strongly with the Msp proteins of T. denticola ATCC 35404 and T. vincentii, reacted very weakly with the Msp protein of T. denticola ATCC 33520, and did not react with T. denticola OTK, T. socranskii, and T. pectinovorum. The msp loci of the T. denticola strains and T. vincentii were identified in analyses using PCR with oligonucleotide primers derived from the DNA sequence Banking msp in ATCC 35405, Southern blot analysis showed at least three groups of related msp DNA sequences, Comparison of DNA sequences of the 5' and 3' ends of the msp genes showed high sequence homolog in the flanking regions and signal peptide coding regions while the homologies between regions encoding the mature peptide were as low as 50%. The entire msp DNA sequences of T. denticola ATCC 33520 and OTK were determined, and the deduced Msp amino acid sequences were compared to the sequence of the previously reported Msp of ATCC 35405, The results show that the msp locus is conserved in oral treponemes but that there are significant differences between the mature msp peptides of different strains, Further studies of the antigenic domains, functional domains, and physical structures of Msp proteins, based on these results, will enhance understanding of the role of Msp in the cytopathology associated with oral spirochetes.