Design and Fabrication of CD-like Microfluidic Platforms for Diagnostics: Microfluidic Functions
Design and Fabrication of CD-like Microfluidic Platforms for Diagnostics: Microfluidic Functions
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DOI：
10.1023/a:1011419515576
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发表时间：
2001-09-01
影响因子：
2.8
通讯作者：
Shih, Chih-Hsin
中科院分区：
文献类型：
--
作者：
Madou, Marc J.;Lee, L. James;Shih, Chih-Hsin
本文介绍了一种基于聚合物的微流控光盘平台的设计。通过平衡离心力和毛细管力，可以将几种微流体功能，如流动测序、级联微混合和毛细管计量集成到CD中。实验证明了这些功能。对于流量排序，使用两点校准设计作为示例，以显示如何通过CD的旋转速度精确控制流体的释放和流动。对于级联微量混合，典型的应用是重构冻干蛋白质。本文还介绍了一种简单的基于气泡在两相流中脱落的计量技术。
In this paper, the design of a polymer based microfluidic compact disk (CD) platform is presented. Several microfluidic functions such as flow sequencing, cascade micro-mixing, and capillary metering can be integrated into the CD by balancing the centrifugal force and the capillary force. These functions are demonstrated experimentally. For flow sequencing, a two-point calibration design is used as an example to show how the release and flow of fluids can be precisely controlled by the rotation speed of the CD. For cascade micro-mixing, a typical application is reconstituting lyophilized protein. A simple metering technique based on bubble snap-off in the two-phase flow is also described.