Defining the anatomical localisation of subsets of the murine mononuclear phagocyte system using integrin alpha X (Itgax, CD11c) and colony stimulating factor 1 receptor (Csf1r, CD115) expression fails to discriminate dendritic cells from macrophages
Defining the anatomical localisation of subsets of the murine mononuclear phagocyte system using integrin alpha X (Itgax, CD11c) and colony stimulating factor 1 receptor (Csf1r, CD115) expression fails to discriminate dendritic cells from macrophages
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DOI：
10.1016/j.imbio.2011.08.006
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发表时间：
2011-11-01
期刊：
影响因子：
2.8
通讯作者：
Mabbott, Neil A.
中科院分区：
文献类型：
--
作者：
Bradford, Barry M.;Sester, David P.;Mabbott, Neil A.
小鼠单核吞噬细胞（MNP）系统包含多种细胞群，包括单核细胞、树突状细胞（DC）和巨噬细胞。这组细胞来源于髓系造血谱系，表达多种特征明确的表面标志物。整合素αX（Itgax，CD11c）的表达通常用于识别经典树突状细胞，类似地，集落刺激因子1受体（Csf1r，CD115）的表达用于识别巨噬细胞。我们利用多种转基因小鼠模型对这些标志物的表达进行了表征。我们证实了先前关于在次级淋巴器官中解剖学上明确的MNP亚群中Itgax表达的观察结果，包括在生发中心内识别出的所有MNP。肠道固有层和肺中的大多数MNP表达Itgax。所有表达Itgax的黏膜细胞也表达Csf1r，这表明其来源于依赖Csf1的造血过程。这种双阳性细胞群包括生发中心的MNP。这些数据表明，仅Itgax表达并不能特异性地定义经典树突状细胞。这些结果提示，对于依赖Itgax的实验以及将其直接等同于独特的由DC介导的活动应更谨慎地解读，特别是在肠道固有层内非淋巴样MNP的功能方面。（C）2011爱思唯尔股份公司。保留所有权利。
The murine mononuclear phagocyte (MNP) system comprises a diverse population of cells, including monocytes, dendritic cells (DC) and macrophages. Derived from the myeloid haematopoietic lineage, this group of cells express a variety of well characterized surface markers. Expression of the integrin alpha X (Itgax, CD11c) is commonly used to identify classical DC, and similarly expression of colony stimulating factor 1 receptor (Csf1r, CD115) to identify macrophages. We have characterized the expression of these markers using a variety of transgenic mouse models. We confirmed previous observations of ltgax expression in anatomically defined subsets of MNPs in secondary lymphoid organs, including all MNPs identified within the germinal centres. The majority of MNPs in the intestinal lamina propria and lung express Itgax. All mucosal Itgax expressing cells also express Csf1r suggesting Csf1-dependent haematopoietic derivation. This double-positive population included germinal centre MNPs. These data reveal that Itgax expression alone does not specifically define classical DC. These results suggest more cautious interpretation of ltgax-dependent experimentation and direct equation with uniquely DC-mediated activities, particularly in the functioning of non-lymphoid MNPs within the intestinal lamina propria. (C) 2011 Elsevier GmbH. All rights reserved.