Effect of Cold Atmospheric Pressure Plasma on Maize Seeds: Enhancement of Seedlings Growth and Surface Microorganisms Inactivation
Effect of Cold Atmospheric Pressure Plasma on Maize Seeds: Enhancement of Seedlings Growth and Surface Microorganisms Inactivation
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DOI：
10.1007/s11090-018-9913-3
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发表时间：
2018-09-01
影响因子：
3.6
通讯作者：
Cernak, Mirko
中科院分区：
文献类型：
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作者：
Zahoranova, Anna;Hoppanova, Lucia;Cernak, Mirko
研究了由扩散共面表面阻挡放电（DCSBD）产生的冷大气压环境空气等离子体对玉米表面原生微生物群和潜在危险病原体（黄曲霉、链格孢菌和黄色镰刀菌）的抑制作用。此外，评价了玉米种子萌发和生长参数的改善。玉米（Zea mays L.; CV. Ronaldinio）是世界上最重要的栽培作物之一，被选为研究材料。电测量证实了DCSBD等离子体的高体积功率密度（80 W cm（-3））。使用光学发射光谱评估的非平衡等离子体状态分别显示振动和旋转温度值（2700 +/- 300）K和（370 +/- 75）K。通过水接触角测定、扫描电镜分析和傅里叶变换红外光谱分析，研究了等离子体处理后种子表面的变化。在60 s（细菌）和180 s（丝状真菌）的短处理时间后，观察到种子表面上的天然微生物群完全失活。人工污染玉米种子的等离子体处理效率估计为F.等离子体处理60 s后，A. culmorum的菌落数为4.21 log（CFU/g）。flavus和A.在300秒的等离子体处理后，此外，所得结果显示润湿性增加，从而导致更好的吸水性和生长参数的增强。所研究的DCSBD等离子体源提供了显着的技术优势和应用潜力的种子表面整理，而不使用危险化学品。
Cold atmospheric pressure ambient air plasma generated by Diffuse Coplanar Surface Barrier Discharge (DCSBD) was investigated for inhibition of native microbiota and potentially dangerous pathogens (Aspergillus flavus, Alternaria alternata and Fusarium culmorum) on the maize surface. Moreover, the improvement of germination and growth parameters of maize seeds was evaluated. Maize (Zea mays L.; cv. Ronaldinio), one of the most important cultivated crops worldwide, was selected as the research material. Electrical measurements confirmed the high volume power density (80 W cm(-3)) of DCSBD plasma. Non-equilibrium plasma state evaluated using optical emission spectroscopy showed values of vibrational and rotational temperature (2700 +/- 300) K and (370 +/- 75) K, respectively. Changes on the plasma treated seeds surface were studied by water contact angle measurement, scanning electron microscope analysis and Fourier transform infrared spectroscopy. A complete devitalisation of native microbiota on the surface of seeds was observed after a short treatment time of 60 s (bacteria) and 180 s (filamentous fungi). The plasma treatment efficiency of artificially contaminated maize seeds was estimated as a reduction of 3.79 log (CFU/g) in F. culmorum after a 60-s plasma treatment, 4.21 log (CFU/g) in A. flavus and 3.22 log (CFU/g) in A. alternata after a 300-s plasma treatment. Moreover, the obtained results show an increase in wettability, resulting in a better water uptake and in an enhancement of growth parameters. The investigated DCSBD plasma source provides significant technical advantages and application potential for seed surface finishing without the use of hazardous chemicals.