Divalent magnesium restores cytoskeletal storage lesions in cold-stored platelet concentrates.
Divalent magnesium restores cytoskeletal storage lesions in cold-stored platelet concentrates.
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DOI：
10.1038/s41598-022-10231-x
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发表时间：
2022-04-14
影响因子：
4.6
通讯作者：
Palankar, Raghavendra
中科院分区：
文献类型：
--
作者：
Aurich, Konstanze;Wesche, Jan;Ulbricht, Martin;Otto, Oliver;Greinacher, Andreas;Palankar, Raghavendra
由于降低了细菌增殖的风险，血小板浓缩物（PC）的冷藏已变得有吸引力，但冷藏血小板的体内循环时间减少。在< 15 °C的温度下，储存细胞器的Ca 2+释放和Ca 2+泵的较高活性触发细胞骨架变化。这被Mg 2+抑制，避免了Ca 2+止血和细胞骨架改变的转变。我们报告了2-10 mM Mg 2+对室温（RT）或4 °C下储存在添加剂溶液（PAS）（30%血浆）中的PC血小板细胞骨架变化的影响。通过实时变形性细胞术（RT-DC）（一种用于生物力学细胞表征的方法）评估血小板的变形。变形受到在4 °C下储存的强烈影响，并且通过Mg 2+添加≥ 4 mM Mg 2+来保持（4 °C对4 °C + IOmM Mg 2+的中值变形的平均值土SD为0.073 ± 0.021对0.118 ± 0.023，p < 0.01; n = 6，第7天）。这些结果通过免疫荧光显微镜证实，表明Mg 2 + ≥ 4 mM可防止4 °C储存诱导的细胞骨架结构损伤。标准的体外血小板功能测试显示RT和冷藏血小板之间存在微小差异。低渗休克反应在RT储存（56.38 ± 29.36%）和含镁（55.22 ± 11.16%）或不含镁（45.65 ± 11.59%; p = 0.042，所有n = 6，第1天）的冷储存血小板之间无显著差异。在室温和4 °C储存的血小板之间，CD 62 P表达和血小板聚集反应相似，在存在较高Mg 2+浓度的情况下变化较小。总之，将PAS中的Mg 2+增加至10 mM抵消了血小板中的4 °C储存损伤，保持了血小板细胞骨架的完整性和与RT储存的血小板相当的生物力学性质。
Cold storage of platelet concentrates (PC) has become attractive due to the reduced risk of bacterial proliferation, but in vivo circulation time of cold-stored platelets is reduced. Ca2+ release from storage organelles and higher activity of Ca2+ pumps at temperatures < 15 °C triggers cytoskeleton changes. This is suppressed by Mg2+ addition, avoiding a shift in Ca2+ hemostasis and cytoskeletal alterations. We report on the impact of 2–10 mM Mg2+ on cytoskeleton alterations of platelets from PC stored at room temperature (RT) or 4 °C in additive solution (PAS), 30% plasma. Deformation of platelets was assessed by real-time deformability cytometry (RT-DC), a method for biomechanical cell characterization. Deformation was strongly affected by storage at 4 °C and preserved by Mg2+ addition ≥ 4 mM Mg2+ (mean ± SD of median deformation 4 °C vs. 4 °C + 10 mM Mg2+ 0.073 ± 0.021 vs. 0.118 ± 0.023, p < 0.01; n = 6, day 7). These results were confirmed by immunofluorescence microscopy, showing that Mg2+ ≥ 4 mM prevents 4 °C storage induced cytoskeletal structure lesion. Standard in vitro platelet function tests showed minor differences between RT and cold-stored platelets. Hypotonic shock response was not significantly different between RT stored (56.38 ± 29.36%) and cold-stored platelets with (55.22 ± 11.16%) or without magnesium (45.65 ± 11.59%; p = 0.042, all n = 6, day 1). CD62P expression and platelet aggregation response were similar between RT and 4 °C stored platelets, with minor changes in the presence of higher Mg2+ concentrations. In conclusion, increasing Mg2+ up to 10 mM in PAS counteracts 4 °C storage lesions in platelets, maintains platelet cytoskeletal integrity and biomechanical properties comparable to RT stored platelets.
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影响因子：
4.6
作者：
Guzniczak E;Mohammad Zadeh M;Dempsey F;Jimenez M;Bock H;Whyte G;Willoughby N;Bridle H
通讯作者：
Bridle H
影响因子：
2.7
作者：
Diedrich, B.;Sandgren, P.;Shanwell, A.
通讯作者：
Shanwell, A.
影响因子：
2.9
作者：
Getz, Todd M.;Turgeon, Annette;Wagner, Stephen J.
通讯作者：
Wagner, Stephen J.
DOI：
10.1161/atvbaha.117.310062
发表时间：
2017-12
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Chen W;Druzak SA;Wang Y;Josephson CD;Hoffmeister KM;Ware J;Li R
通讯作者：
Li R
DOI：
10.1016/j.transci.2009.10.008
发表时间：
2010-02
期刊：
Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis
影响因子：
--
作者：
Rumjantseva V;Hoffmeister KM
通讯作者：
Hoffmeister KM