The bacterial second messenger cyclic diGMP exhibits potent adjuvant properties
The bacterial second messenger cyclic diGMP exhibits potent adjuvant properties
复制标题
DOI：
10.1016/j.vaccine.2006.10.033
复制
发表时间：
2007-02-09
期刊：
影响因子：
5.5
通讯作者：
Guzman, Carlos A.
中科院分区：
文献类型：
--
作者：
Ebensen, Thomas;Schulze, Kai;Guzman, Carlos A.
新佐剂的鉴定是疫苗学的一个关键需求。在这项工作中，证明了二-(3',5')-环二聚鸟苷单磷酸（cdiGMP）具有有效的佐剂特性。小鼠皮下注射cdiGMP和p -半乳糖苷酶（P-Gal）后，血清中抗原特异性IgG滴度明显高于单独注射P-Gal的小鼠。在免疫动物中，P-Gal蛋白和包含其MHC i类限制性表位的肽也会产生强烈的细胞免疫反应，其特征是Th1/Th2模式平衡。这些结果表明，cdiGMP是一种很有希望用于疫苗开发的佐剂。(c) 2006 Elsevier Ltd.版权所有。
The identification of new adjuvants is a critical need in vaccinology. In this work, it is demonstrated that bis-(3',5')-cyclic dimeric guanosine monophosphate (cdiGMP) exhibits potent adjuvant properties. Subcutaneous co-administration of cdiGMP with P-galactosidase (P-Gal) to mice resulted in the elicitation of significantly higher antigen-specific serum IgG titres than in animals receiving P-Gal alone. Strong cellular immune responses, which were characterized by a balanced Th1/Th2 pattern, were also observed in response to the P-Gal protein and a peptide encompassing its MHC class I-restricted epitope in immunized animals. These results suggest that cdiGMP represents a promising adjuvant for vaccine development. (c) 2006 Elsevier Ltd. All rights reserved.