Domestication of Campylobacter jejuni NCTC 11168
Domestication of Campylobacter jejuni NCTC 11168
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空肠弯曲菌的驯化 NCTC 11168
DOI：
10.1101/591701
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Pascoe B
中科院分区：
文献类型：
--
作者：
Pascoe B
几十年来，已知细菌的参考菌株和模式菌株一直是微生物学研究的基石。在实验室之间共享良好表征的分离物与研究工作并行进行，并增强了实验的可重复性，从而产生了关于不同物种的性状变异和潜在遗传学的丰富知识。已被世界各地的研究人员广泛采用作为参考菌株，并且是第一个完整基因组被公布的弯曲杆菌（2000年）。在这项研究中，我们收集了23 C. jejuniNCTC 11168参考分离株从英国各地的实验室，并比较了简单的实验室表型的变异与基因组测序的遗传变异。与最近的参考菌株相比，先前鉴定为具有异常表型的脓毒症相同分离株的变化高达281个SNP（在15个基因中）。分离株在运动性、形态、37 °C下的生长、鸡和人细胞系的侵袭以及对氨苄青霉素的敏感性方面也显示出相当大的表型变异。这项研究提供了正在进行的进化变化的证据。jejunisolates在不同的实验室培养，并强调需要仔细考虑实验室参考菌株的遗传变异。本文包含由Microreact托管的数据。
Reference and type strains of well-known bacteria have been a cornerstone of microbiology research for decades. The sharing of well-characterized isolates among laboratories has run in parallel with research efforts and enhanced the reproducibility of experiments, leading to a wealth of knowledge about trait variation in different species and the underlying genetics.Campylobacter jejunistrain NCTC 11168, deposited at the National Collection of Type Cultures in 1977, has been adopted widely as a reference strain by researchers worldwide and was the firstCampylobacterfor which the complete genome was published (in 2000). In this study, we collected 23C.jejuniNCTC 11168 reference isolates from laboratories across the UK and compared variation in simple laboratory phenotypes with genetic variation in sequenced genomes. Putatively identical isolates, identified previously to have aberrant phenotypes, varied by up to 281 SNPs (in 15 genes) compared to the most recent reference strain. Isolates also display considerable phenotype variation in motility, morphology, growth at 37 °C, invasion of chicken and human cell lines, and susceptibility to ampicillin. This study provides evidence of ongoing evolutionary change amongC. jejuniisolates as they are cultured in different laboratories and highlights the need for careful consideration of genetic variation within laboratory reference strains. This article contains data hosted by Microreact.