Misexpression screen for genes altering the olfactory map in Drosophila.
Misexpression screen for genes altering the olfactory map in Drosophila.
复制标题
改变果蝇嗅觉图谱的基因的错误表达筛选。
DOI：
10.1002/dvg.20202
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
Hing,Huey
中科院分区：
文献类型：
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作者：
Zhang,Dongsheng;Zhou,Weiguang;Yin,Chong;Chen,Weitao;Ozawa,Rie;Ang,Lay-Hong;Anandan,Lavanya;Aigaki,Toshiro;Hing,Huey
尽管已经识别出了一些引导分子，但还没有一个全面的图片来解释嗅觉地图的精确解剖。从1,515 P {GS}细胞系的错误表达筛选中，我们鉴定出23个基因，当它们在嗅觉受体神经元中强制表达时，会破坏果蝇触角叶的刻板解剖结构。这些基因，这还没有被证明以前控制嗅觉地图的发展，编码新的蛋白质，以及已知的轴突生长和细胞骨架重塑的蛋白质。我们分析了Akap 200，它编码一种蛋白激酶A结合蛋白。Akap 200的过度表达导致肾小球融合，而其缺失导致畸形和异位肾小球。Akap 200的需要验证了我们的筛选作为恢复控制肾小球图谱模式的基因的有效方法。我们发现的不同类别的基因揭示了复杂的机制，嗅觉地图的发展。创世纪44：189-201，2006。2006年出版Wiley利斯公司
Despite the identification of a number of guidance molecules, a comprehensive picture has yet to emerge to explain the precise anatomy of the olfactory map. From a misexpression screen of 1,515P{GS}lines, we identified 23 genes that, when forcibly expressed in the olfactory receptor neurons, disrupted the stereotyped anatomy of theDrosophilaantennal lobes. These genes, which have not been shown previously to control olfactory map development, encode novel proteins as well as proteins with known roles in axonal outgrowth and cytoskeletal remodeling. We analyzedAkap200, which encodes a Protein Kinase A‐binding protein. Overexpression ofAkap200resulted in fusion of the glomeruli, while its loss resulted in misshapen and ectopic glomeruli. The requirement ofAkap200validates our screen as an effective approach for recovering genes controlling glomerular map patterning. Our finding of diverse classes of genes reveals the complexity of the mechanisms that underlie olfactory map development. genesis 44:189–201, 2006. Published 2006 Wiley‐Liss, Inc.