Development of a microfluidic biosensor module for pathogen detection
Development of a microfluidic biosensor module for pathogen detection
复制标题
DOI：
10.1039/b503856a
复制
发表时间：
2005-01-01
期刊：
影响因子：
6.1
通讯作者：
Baeumner, AJ
中科院分区：
文献类型：
--
作者：
Zaytseva, NV;Goral, VN;Baeumner, AJ
本文介绍了用于识别病原生物和病毒的具有荧光检测功能的微流体生物传感器模块的开发。微流体生物传感器由在聚二甲基硅氧烷 (PDMS) 基底上制造的微通道网络组成。微通道用玻璃基板密封并封装在有机玻璃外壳中，以提供与宏观世界的连接并确保无泄漏的流动操作。可逆密封允许轻松拆卸以清洁和更换微流体通道。流体流动是通过施加正压梯度产生的，并且该模块可以在高达 80 μL min(-1) 的连续溶液流下运行。生物传感器识别原理基于DNA/RNA杂交和脂质体信号放大。超顺磁珠作为移动固体支持物纳入系统中，是分析方案的重要组成部分。在这项研究中，进行了不同生物传感器组件的设计、制造以及浓度和数量的优化。包括样品孵育时间在内，检测所需的总时间仅为 15 分钟。该生物传感器模块的设计使其可以轻松地与微型整体分析系统集成，该系统将样品制备和检测步骤结合到单个芯片上。
The development of a microfluidic biosensor module with fluorescence detection for the identification of pathogenic organisms and viruses is presented in this article. The microfluidic biosensor consists of a network of microchannels fabricated in polydimethylsiloxane ( PDMS) substrate. The microchannels are sealed with a glass substrate and packed in a Plexiglas housing to provide connection to the macro-world and ensure leakage-free flow operation. Reversible sealing permits easy disassembly for cleaning and replacing the microfluidic channels. The fluidic flow is generated by an applied positive pressure gradient, and the module can be operated under continuous solution flow of up to 80 mu L min(-1). The biosensor recognition principle is based on DNA/RNA hybridization and liposome signal amplification. Superparamagnetic beads are incorporated into the system as a mobile solid support and are an essential part of the analysis scheme. In this study, the design, fabrication and the optimization of concentrations and amounts of the different biosensor components are carried out. The total time required for an assay is only 15 min including sample incubation time. The biosensor module is designed so that it can be easily integrated with a micro total analysis system, which will combine sample preparation and detection steps onto a single chip.