Genomic characterization of a temperate phage of the psychrotolerant deep-sea bacterium Aurantimonas sp.
Genomic characterization of a temperate phage of the psychrotolerant deep-sea bacterium Aurantimonas sp.
复制标题
DOI：
10.1007/s00792-014-0702-5
复制
发表时间：
2014
期刊：
影响因子：
2.9
通讯作者：
Mitsuhiro Yoshida;Yukari Yoshida-Takashima;T. Nunoura;K. Takai
中科院分区：
文献类型：
--
作者：
Mitsuhiro Yoshida;Yukari Yoshida-Takashima;T. Nunoura;K. Takai
从耐冷根瘤菌目细菌（Rhizobiales bacteriumAurantimonassp）中鉴定出一株温和噬菌体（AmM-1）。C5-1，分离自西北太平洋深海层水（水深= 1 500米）。AmM-1基因组长度为47，800 bp，包含67个编码序列。虽然噬菌体AmM-1在形态上属于Myoviridae，但其基因组结构，特别是模块化基因组组织，类似于Siphoviridae的Escherida型Escherichia。结构核心基因的遗传和系统发育分析还显示，AmM-1具有镶嵌的基因组结构，包括一个类似于Escherida的头（Siphoviridae）和P2-样的尾（Myoviridae）。AmM-1的结构核心基因的序列不同于先前表征的噬菌体群，但与最近鉴定的海洋根瘤菌目细菌的一个原噬菌体元件和一个噬菌体相似：海洋耐冷AureimonasureilyticaDSM 18598基因组中的一个潜在的原噬菌体元件和从深海底沉积物中分离的感染放射性根瘤菌P007的温带噬菌体RR-1A。AmM-1的嵌合基因组结构表明海洋根瘤菌目中不同温带类群之间发生了遗传交换。
A temperate phage (termed AmM-1) was identified from the psychrotolerant Rhizobiales bacteriumAurantimonassp. C5-1, which was isolated from bathypelagic water (water depth = 1,500 m) in the northwest Pacific. The AmM-1 genome is 47,800 bp in length and contains 67 coding sequences. Although phage AmM-1 morphologically belongs to the familyMyoviridae, its genomic structure, particularly modular genome organization, is similar to that of lambda-type phages ofSiphoviridae. Genetic and phylogenetic analyses of the structural core genes also revealed that AmM-1 has a mosaic genomic structure that includes a lambda-like head (Siphoviridae) and P2-like tail (Myoviridae). The sequences of the structural core genes of AmM-1 are distinct from those of previously characterized phage groups but similar to those of recently identified one prophage element and one phage of marine Rhizobiales bacteria: a potential prophage element in the marine psychrotolerantAureimonas ureilyticaDSM 18598 genome and the temperate phage RR-1A infectingRhizobium radiobacterP007 isolated from deep subseafloor sediment. The mosaic genome structure of AmM-1 suggests the occurrence of genetic exchange between distinct temperate phages in marine Rhizobiales populations.