A rapid, accurate, immunohistochemical method to label proliferating cells in the digestive tract. A comparison with tritiated thymidine.
A rapid, accurate, immunohistochemical method to label proliferating cells in the digestive tract. A comparison with tritiated thymidine.
复制标题
一种快速、准确的免疫组织化学方法，用于标记消化道中的增殖细胞。
DOI：
10.1016/0016-5085(91)90610-w
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发表时间：
1991
期刊：
影响因子：
29.4
通讯作者：
Sistrunk,S
中科院分区：
文献类型：
--
作者：
Lacy,ER;Kuwayama,H;Cowart,KS;King,JS;Deutz,AH;Sistrunk,S
用5′-溴-2 ′-脱氧尿苷（BrdU）标记增殖上皮细胞，并与标准氚标记的胸苷放射自显影（3 H-TdR）对照，在大鼠食管、胃、十二指肠和结肠中进行了快速免疫组化标记。IP注射BrdU或3 H-胸苷或两种化合物（同时）。用单克隆抗BrdU抗体对这些组织的组织切片进行免疫染色，通过标准抗生物素蛋白-生物素技术与辣根过氧化物酶连接，并用二氨基联苯胺染色，或将切片浸渍用于放射自显影，或将两种技术应用于同一组织切片。结果表明：（a）BrdU标记的增殖细胞数与~ 3 H-TdR相同;（B）BrdU与~ 3 H-TdR共标记;（c）BrdU在3-4天内完成免疫染色，而标准~ 3 H-TdR需要2周;（d）BrdU定性分析所需时间比标准~ 3 H-TdR少50%。
A rapid immunohistochemical method using 5′-bromo-2′-deoxyuridine (BrdU), a thymidine analog, for labeling proliferating epithelial cells was modified and tested for accuracy against standard tritiated thymidine autoradiography (3H-TdR) in rat esophagus, stomach, duodenum, and colon. Either BrdU or3H-thymidine or both compounds (simultaneously) were injected IP. Histological sections of these tissues were immunostained with monoclonal anti-BrdU antibody, linked to horseradish peroxidase by standard avidin-biotin techniques and stained with diaminobenzidine, or sections were dipped for autoradiography, or both techniques were applied to the same tissue section. Results showed that (a) BrdU labeled the same number of proliferating cells in all organs as3H-TdR; (b) BrdU colabeled with3H-thymidine; (c) immunostaining was complete in 3–4 days but standard3H-TdR took 2 weeks; and (d) qualitative analysis took 50% less time with BrdU than with standard3H-TdR.