Distinct roles of the pepper hypersensitive induced reaction protein gene CaHIR1 in disease and osmotic stress, as determined by comparative transcriptome and proteome analyses
Distinct roles of the pepper hypersensitive induced reaction protein gene CaHIR1 in disease and osmotic stress, as determined by comparative transcriptome and proteome analyses
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DOI：
10.1007/s00425-007-0628-6
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发表时间：
2008-01-01
期刊：
影响因子：
4.3
通讯作者：
Hwang, Byung Kook
中科院分区：
文献类型：
--
作者：
Jung, Ho Won;Lim, Chae Woo;Hwang, Byung Kook
最近提出辣椒过敏诱导反应蛋白 1 (CaHIR1) 作为植物过敏细胞死亡的正调节因子。 CaHIR1在转基因拟南芥植物中的过度表达增强了对半生物营养型丁香假单胞菌的抗性。番茄 (Pst) 和生物营养型寄生透明霜霉 (Hyaloperonospora parasitica)。携带avrRpm1或avrRpt2的无毒假单胞菌菌株的感染导致转基因植物的抗性反应增强，这表明CaHIR1以非种族特异性的方式参与基础抗病性。 H. parasitica 在 CaHIR1 幼苗上表现出低水平的无性孢子形成。相比之下，转基因植物不仅对坏死性真菌病原体灰葡萄孢敏感，而且对高盐度和干旱引起的渗透胁迫也敏感。为了鉴定拟南芥叶片中因 CaHIR1 过表达而改变表达的蛋白质，使用二维凝胶电泳与质谱联用进行了定量比较蛋白质组分析。在大约 400 种可溶性蛋白质中，有 11 种参与多种代谢途径的蛋白质因 CaHIR1 过表达而上调或下调。编码甘氨酸脱羧酶 (At2g35370) 和一种未知蛋白 (At2g03440) 的基因在 CaHIR1 过表达的拟南芥中强烈上调，也通过 Pst 感染在转录水平上差异诱导。拟南芥碳酸酐酶 (At3g01500) 与烟草水杨酸结合蛋白 3 高度相似，CaHIR1 过表达会上调。在转基因拟南芥中，抗氧化酶铜/锌超氧化物歧化酶 (At2g28190) 的活性也因 CaHIR1 过表达而减弱。总之，这些结果表明 CaHIR1 在拟南芥中的过表达介导植物对生物营养型、半生物营养型和坏死营养型病原体以及不同方式的渗透胁迫的反应。
A Capsicum annuum hypersensitive induced reaction protein1 (CaHIR1) was recently proposed as a positive regulator of hypersensitive cell death in plants. Overexpression of CaHIR1 in transgenic Arabidopsis plants conferred enhanced resistance against the hemi-biotrophic Pseudomonas syringae pv. tomato (Pst) and the biotrophic Hyaloperonospora parasitica. Infection by avirulent Pseudomonas strains carrying avrRpm1 or avrRpt2 caused enhanced resistance responses in transgenic plants, suggesting that CaHIR1 is involved in basal disease resistance in a race-nonspecific manner. H. parasitica exhibited low levels of asexual sporulation on CaHIR1 seedlings. In contrast, transgenic plants were susceptible not only to the necrotrophic fungal pathogen Botrytis cinerea but were also sensitive to osmotic stress caused by high salinity and drought. To identify proteins whose expression was altered by CaHIR1 overexpression in Arabidopsis leaves, a quantitative comparative proteome analysis using two-dimensional gel electrophoresis coupled with mass spectrometry was performed. Of about 400 soluble proteins, 11 proteins involved in several metabolic pathways were up- or down-regulated by CaHIR1 overexpression. Genes encoding glycine decarboxylase (At2g35370) and an unidentified protein (At2g03440), which were strongly upregulated in CaHIR1-overexpressing Arabidopsis, were also differentially induced at the transcriptional level by Pst infection. Arabidopsis carbonic anhydrase (At3g01500), highly similar to tobacco salicylic acid-binding protein 3, was up-regulated by CaHIR1 overexpression. The activity of an anti-oxidant enzyme, cooper/zinc superoxide dismutase (At2g28190), was also attenuated in transgenic Arabidopsis by CaHIR1 overexpression. Together, these results suggest that CaHIR1 overexpression in Arabidopsis mediates plant responses to biotrophic, hemi-biotrophic and necrotrophic pathogens, as well as to osmotic stress in different ways.