Endostar Combined with Cisplatin Inhibits Tumor Growth and Lymphatic Metastasis of Lewis Lung Carcinoma Xenografts in Mice
Endostar Combined with Cisplatin Inhibits Tumor Growth and Lymphatic Metastasis of Lewis Lung Carcinoma Xenografts in Mice
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DOI：
10.7314/apjcp.2013.14.5.3079
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发表时间：
2013-01-01
影响因子：
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通讯作者：
Zhao, Xiao-Gang
中科院分区：
文献类型：
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作者：
Dong, Xiao-Peng;Xiao, Tian-Hui;Zhao, Xiao-Gang
目的：目的探讨重组人源化血管内皮抑素（endostar）联合顺铂对小鼠刘易斯肺癌（LLC）生长、淋巴管生成和淋巴道转移的影响。方法：采用LLC细胞静脉移植法建立C57 BL/6小鼠肿瘤模型。然后将小鼠随机接受NS、恩度、顺铂或恩度+顺铂给药。处死小鼠后，评估肿瘤多样性、肿瘤大小和淋巴结转移。免疫组化法检测血管内皮生长因子-C（VEGF-C）和podoplanin的表达。结果：恩度联合顺铂能明显抑制肿瘤生长。淋巴结转移和延长小鼠生存时间，无明显毒性。淋巴结转移的抑制与微淋巴管密度（MLVD）和VEGF-C表达的降低有关。结论：恩度联合顺铂对肿瘤生长和淋巴结转移的抑制作用优于单独用药。这可能为肺癌的治疗提供一种合理的选择。
Objective: To investigate the effects of endostar, a recombined humanized endostatin, plus cisplatin on the growth, lymphangiogenesis and lymphatic metastasis of the Lewis lung carcinoma (LLC) in mice. Methods: A tumor model were established in C57BL/6 mice by intravenious transplantation of LLC cells. Then the mice were randomized to receive administration with NS, endostar, cisplatin, or endostar plus cisplatin. After the mice were sacrificed, tumor multiplicity, tumor size and lymph node metastasis were assessed. Then the expression of vascular endothelial growth factor-c (VEGF-C) and podoplanin were determined by immunohistochemical staining. Results: Endostar plus cisplatin significantly suppressed tumor growth. lymphatic metastasis and prolonged survival time of the mice without obvious toxicity. The inhibition of lymphatic metastasis was associated with decreased microlymphatic vessel density (MLVD) and expression of VEGF-C. Conclusions: Endostar combined with cisplatin was more effective to suppress tumor growth and lymphatic metastasis than either agent alone. Thus this may provide a rational alternative for lung carcinoma treatment.